Laboratory of Functional and Metabolic Imaging, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Neuroscience Research Center, Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland.
Sci Rep. 2021 Mar 11;11(1):5771. doi: 10.1038/s41598-021-85339-7.
Glioblastoma (GBM) is the most aggressive brain tumor type in adults. GBM is heterogeneous, with a compact core lesion surrounded by an invasive tumor front. This front is highly relevant for tumor recurrence but is generally non-detectable using standard imaging techniques. Recent studies demonstrated distinct metabolic profiles of the invasive phenotype in GBM. Magnetic resonance (MR) of hyperpolarized C-labeled probes is a rapidly advancing field that provides real-time metabolic information. Here, we applied hyperpolarized C-glucose MR to mouse GBM models. Compared to controls, the amount of lactate produced from hyperpolarized glucose was higher in the compact GBM model, consistent with the accepted "Warburg effect". However, the opposite response was observed in models reflecting the invasive zone, with less lactate produced than in controls, implying a reduction in aerobic glycolysis. These striking differences could be used to map the metabolic heterogeneity in GBM and to visualize the infiltrative front of GBM.
胶质母细胞瘤（GBM）是成人中最具侵袭性的脑肿瘤类型。GBM 具有异质性，在致密的核心病变周围有一个侵袭性的肿瘤前缘。这个前缘与肿瘤复发高度相关，但通常无法用标准的成像技术检测到。最近的研究表明，GBM 的侵袭表型具有独特的代谢特征。¹H 磁共振（MR）检测 ¹³C 标记探针是一个快速发展的领域，可提供实时代谢信息。在这里，我们应用¹³C-葡萄糖的极化磁共振来检测小鼠 GBM 模型。与对照组相比，在致密 GBM 模型中，从极化葡萄糖中产生的乳酸量更高，这与公认的“Warburg 效应”一致。然而，在反映侵袭区的模型中观察到相反的反应，产生的乳酸比对照组少，这意味着有氧糖酵解减少。这些显著的差异可用于绘制 GBM 的代谢异质性，并可视化 GBM 的浸润前沿。
