ICAR-Central Institute of Fisheries Education, Mumbai, 400 061, India.
College of Fisheries, Central Agricultural University, Lembucherra, Tripura, 799210, India.
Sci Rep. 2021 Mar 11;11(1):5713. doi: 10.1038/s41598-021-85311-5.
Transportation of fish seed is a complex phenomenon associated with multiple kinds of stressors that simultaneously affect the fish in a confined environment, causing stress and mortality. The present study investigated the stress-relieving effect of exogenous glucose as a water additive in different concentrations (0.1, 0.2, 0.3, and 0.4%) during simulated transportation (12 h) of L. rohita fingerlings. The integrated biomarker response (IBR) index is a holistic tool to determine the optimum dose of exogenous glucose for mitigating transportation stress in fish. Based on selected biomarkers related to the stress hormone, serum biochemistry, oxidative stress, and HSP70 mRNA expression, the IBR index is calculated for each treatment and control group. The result showed a significant change in the level of stress hormone cortisol, enzymes (SGPT, LDH, MDH, SOD, CAT) and metabolites (serum glucose, triglyceride, creatinine) along with an upregulation in liver HSP70 mRNA expression. IBR index suggests that 0.2% glucose exhibited the lowest multi-biomarker stress response in comparison to other treatments and control. Therefore, the use of 0.2% glucose as a water additive will provide a solution to transportation induced stress in L. rohita fingerling and will underwrite the success of grow-out fish culture in days to come.
鱼类苗种运输是一种复杂的现象，涉及多种胁迫因子，这些因子会同时在封闭环境中影响鱼类，导致鱼类产生应激反应和死亡。本研究调查了在模拟运输（12 小时）期间，作为一种水添加剂的外源葡萄糖在不同浓度（0.1%、0.2%、0.3%和 0.4%）下对罗非鱼鱼苗的缓解应激作用。综合生物标志物响应（IBR）指数是一种整体工具，用于确定外源葡萄糖的最佳剂量，以减轻鱼类运输应激。根据与应激激素、血清生化、氧化应激和 HSP70 mRNA 表达相关的选定生物标志物，为每个处理组和对照组计算 IBR 指数。结果表明，应激激素皮质醇、酶（SGPT、LDH、MDH、SOD、CAT）和代谢物（血清葡萄糖、甘油三酯、肌酐）水平显著变化，肝脏 HSP70 mRNA 表达上调。IBR 指数表明，与其他处理组和对照组相比，0.2%葡萄糖表现出最低的多生物标志物应激反应。因此，使用 0.2%葡萄糖作为水添加剂将为罗非鱼鱼苗运输诱导的应激提供解决方案，并为未来的鱼类养殖成功奠定基础。
