Liu Heng, Liu Yu, Cheng Nini, Zhang Yuanhu
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 Dai Zong Street, Tai'an, 271018 Shandong People's Republic of China.
Qingdao Agricultural University, Qingdao, 266109 Shandong People's Republic of China.
Physiol Mol Biol Plants. 2021 Feb;27(2):237-249. doi: 10.1007/s12298-021-00953-z. Epub 2021 Feb 19.
The enzyme, α-farnesene synthase (AFS), which synthesizes α-farnesene, is the final enzyme in α-farnesene synthesis pathway. We overexpressed the α-farnesene synthase gene (previously cloned in our lab from apple peel) and ectopically expressed it in tobacco ( NC89). Then, the transgenic plants showed an accelerated developmental process and bloomed about 7 weeks earlier than the control plants. We anticipate that de novo transcriptomic analyses of may provide useful information on isoprenoid biosynthesis, growth, and development. We generated 318,925,338 bp sequencing data using Illumina paired-end sequencing from the cDNA library of the apical buds of transgenic line and the wild-type line. We annotated and functionally classified the unigenes in a nucleotide and protein database. Differentially expressed unigenes may be involved in carbohydrate metabolism, nitrogen metabolism, transporter activity, hormone signal transduction, antioxidant systems and transcription regulator activity particularly related to senescence. Moreover, we analyzed eight genes related to terpenoid biosynthesis using qRT-PCR to study the changes in growth and development patterns in the transgenic plants. Our study shows that transgenic plants show premature senescence.
The online version contains supplementary material available at 10.1007/s12298-021-00953-z.
合成α-法尼烯的酶α-法尼烯合酶（AFS）是α-法尼烯合成途径中的最终酶。我们对α-法尼烯合酶基因（先前在我们实验室从苹果皮中克隆）进行了过表达，并在烟草（NC89）中异位表达。然后，转基因植物显示出发育过程加速，比对照植物早约7周开花。我们预计对[此处原文缺失部分内容]进行从头转录组分析可能会提供有关类异戊二烯生物合成、生长和发育的有用信息。我们使用Illumina双末端测序从转基因系和野生型系顶芽的cDNA文库中生成了318,925,338 bp的测序数据。我们在核苷酸和蛋白质数据库中对单基因进行了注释和功能分类。差异表达的单基因可能参与碳水化合物代谢、氮代谢、转运蛋白活性、激素信号转导、抗氧化系统以及特别是与衰老相关的转录调节活性。此外，我们使用qRT-PCR分析了八个与萜类生物合成相关的基因，以研究转基因植物生长和发育模式的变化。我们的研究表明转基因植物表现出早衰。
在线版本包含可在10.1007/s12298-021-00953-z获取的补充材料。
