Bai Rui, Wu Dehao, Shi Zhong, Hu Wangxiong, Li Juan, Chen Yuanyuan, Ge Weiting, Yuan Ying, Zheng Shu
Department of Medical Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Chin J Cancer Res. 2021 Feb 28;33(1):93-102. doi: 10.21147/j.issn.1000-9604.2021.01.10.
Ankyrin repeat domain-containing protein 6 (ANKRD6) is an ankyrin repeat-containing protein which is structurally related to vertebrate inversin and Drosophila Diego. However, the correlations between ANKRD6 and tumor-infiltrating immune cells in cancers is not clear.
ANKRD6 expression was analyzed by Oncomine, Tumor Immune Estimation Resource (TIMER) and Gene Expression Profiling Interactive Analysis (GEPIA). PrognoScan and GEPIA were used to evaluate the influence of ANKRD6 on clinical prognosis. TIMER and CIBERSORT were used to analyze correlations between ANKRD6 expression levels and tumor immune cell infiltrates. Immunohistochemical analysis of the relationship between ANKRD6 expression and overall survival, as well as the relationship between ANKRD6 expression and M2 macrophage infiltration, was performed.
High level of ANKRD6 expression was associated with poor prognosis of colon cancer. ANKRD6 expression level was positively correlated with infiltrating levels of CD8+ T cells, CD4+ T cells, macrophages, neutrophils and dendritic cells in colon cancer by using TIMER. Using CIBERSORT, we found that in plasma cells, CD8+ T cells, CD4+ memory resting T cells, follicular helper T cells and activated natural killer cells were significantly lower in the ANKRD6-high group than in the ANKRD6-low group. M0 and M2 macrophages were significantly higher in the ANKRD6-high group than in the ANKRD6-low group. Immunohistochemistry confirmed that M2 macrophage infiltration in the ANKRD6-high group significantly increased.
The high ANKRD6 expression is associated with poor prognosis of colon cancer. ANKRD6 expression is positively correlated with M2 macrophage infiltration in colon cancer.
含锚蛋白重复结构域蛋白6（ANKRD6）是一种含锚蛋白重复结构域的蛋白，在结构上与脊椎动物的inversin和果蝇的Diego相关。然而，ANKRD6与癌症中肿瘤浸润免疫细胞之间的相关性尚不清楚。
通过Oncomine、肿瘤免疫评估资源（TIMER）和基因表达谱交互分析（GEPIA）分析ANKRD6的表达。使用PrognoScan和GEPIA评估ANKRD6对临床预后的影响。使用TIMER和CIBERSORT分析ANKRD6表达水平与肿瘤免疫细胞浸润之间的相关性。进行免疫组织化学分析ANKRD6表达与总生存期之间的关系，以及ANKRD6表达与M2巨噬细胞浸润之间的关系。
ANKRD6高表达与结肠癌预后不良相关。使用TIMER分析发现，ANKRD6表达水平与结肠癌中CD8 + T细胞、CD4 + T细胞、巨噬细胞、中性粒细胞和树突状细胞的浸润水平呈正相关。使用CIBERSORT分析发现，在浆细胞、CD8 + T细胞、CD4 + 记忆静止T细胞、滤泡辅助性T细胞和活化的自然杀伤细胞中，ANKRD6高表达组显著低于ANKRD6低表达组。M0和M2巨噬细胞在ANKRD6高表达组中显著高于ANKRD6低表达组。免疫组织化学证实ANKRD6高表达组中M2巨噬细胞浸润显著增加。
ANKRD6高表达与结肠癌预后不良相关。ANKRD6表达与结肠癌中M2巨噬细胞浸润呈正相关。
