Julien C, Rodriguez C, Sacquet J, Cuisinaud G, Sassard J
Department of Physiology and Clinical Pharmacology, (UA CNRS 606), Faculty of Pharmacy, Lyon, France.
Clin Chem. 1988 May;34(5):966-9.
We describe the conjoint measurement in urine of the alcoholic catecholamine metabolites 3,4-dihydroxyphenylglycol (DHPG) and 3-methoxy-4-hydroxyphenylglycol (MHPG). The method involves extraction with ethyl acetate and back-extraction into acetic acid (MHPG), followed by adsorption onto alumina (DHPG). The separation is performed by reversed-phase liquid chromatography with column switching and amperometric detection. Total (free plus conjugated) compounds are determined after a 24-h enzymatic hydrolysis. Twelve-hour excretion values for normal and hypertensive subjects are given, together with results obtained for patients with pheochromocytoma, chemodectoma, and congenital dopamine-beta-hydroxylase deficiency. The assay is sensitive, reproducible, and free of interferences, and may lead to a better understanding of overall catecholamine metabolism in health and disease.
我们描述了对尿液中酒精性儿茶酚胺代谢产物3,4-二羟基苯乙二醇（DHPG）和3-甲氧基-4-羟基苯乙二醇（MHPG）的联合测量。该方法包括用乙酸乙酯萃取，然后反萃取到乙酸中（MHPG），接着吸附到氧化铝上（DHPG）。通过柱切换反相液相色谱和安培检测进行分离。在24小时酶促水解后测定总（游离加结合）化合物。给出了正常和高血压受试者的12小时排泄值，以及嗜铬细胞瘤、化学感受器瘤和先天性多巴胺-β-羟化酶缺乏症患者的检测结果。该测定方法灵敏、可重复且无干扰，可能有助于更好地理解健康和疾病状态下的整体儿茶酚胺代谢。
