Taylor J T, Freeman S, Brewer P
Clin Chem. 1981 Jan;27(1):173-5.
We describe a liquid-chromatographic procedure for determining low concentrations of 3-methoxy-4-hydroxyphenylethylene glycol in urine. After liquid-liquid extraction and cleanup of the sample, an aliquot of the extract is injected onto a reversed-phase chromatographic column, with isocratic elution with an acetonitrile/acetate buffer mobile phase. An on-line fluorescence detector is used for measuring the natural fluorescence of the compound. The method gives a relatively clean extract, which is suitable for measuring the less-than-normal concentrations associated with mentally depressed patients. Although a liquid-chromatographic technique has been recently described for measuring this compound in urine, the method involves electrochemical detection, which may not be available in many clinical laboratories, and, because of its nonspecificity, the method is only suitable for detecting the increased amounts of 3-methoxy-4-hydroxyphenylethylene glycol excreted by patients with pheochromocytoma.
我们描述了一种用于测定尿液中低浓度3-甲氧基-4-羟基苯乙二醇的液相色谱法。在液-液萃取和样品净化后，取一份萃取液注入反相色谱柱，用乙腈/醋酸盐缓冲液流动相进行等度洗脱。使用在线荧光检测器测量该化合物的天然荧光。该方法得到的萃取液相对纯净，适用于测量与精神抑郁患者相关的低于正常浓度的情况。尽管最近已描述了一种用于测定尿液中该化合物的液相色谱技术，但该方法涉及电化学检测，许多临床实验室可能没有这种设备，而且由于其非特异性，该方法仅适用于检测嗜铬细胞瘤患者排泄的3-甲氧基-4-羟基苯乙二醇增加的量。
