Binandeh Mansour, Karimi Farrokh, Rostamnia Sadegh
Department of Chemistry, Faculty of Science, University of Maragheh, Iran.
Department of Biotechnology and Environmental, Faculty of Science, University of Maragheh, Iran.
Int J Health Sci (Qassim). 2021 Mar-Apr;15(2):3-8.
The project is based on extensive studies on applied nanoparticles in biology and medicine. This study was primarily designed to investigate the role of magnetic nanoparticles by comparing the absorbance and stabilization of DNA-C, L by the electrophoretic detection.
FeO magnetic nanoparticles (MNPs) with core/shell structure of silica coatings were synthesized by a chemical coprecipitation method. This work is done at 15 min times with multitimes, that is, 20 numbers and nanoparticles are provided regular with good morphology which was synthesized in 20 nm in size, and its structure was analyzed by tools such as EDX analysis. Next, 20 mg of the magnetic nanoparticles were coated with silica in a heterogeneous solution at 25 μg/ml of the solution of each of the mixed DNAs (ring, linear) in separate containers. Finally, 15 minutes later, DNA was adsorbed on the surface of the nanoparticles. The amount of this adsorption was injected by spectrophotometry (UV-Vis, ith 99% accuracy and optimized by the standard Tris.HCl buffer required to separate DNA from its pure solution [unabsorbed DNA]) and electrophoresis.
The results showed that absorption and diffusion of DNA-C or L at the surface of nanoparticles were 95% and 85%, respectively (i.e., absorbance of DNA-C>DNA-L is) with rate of removing of on MNPs was >99%. Hence, after review, we received that a linkage of electrostatic bonding between nanoparticles and biomolecules was obtained, and the results of the EDX analysis confirmed this study.
In this project, nanocomposites containing magnetic nanoparticles were synthesized and their structure was identified by relevant analyzes. It was then used to stabilize the biomolecules, which yielded competitive results between the two types of DNA (linear and cyclic) at 85 and 95% adsorption, respectively.
该项目基于对生物学和医学中应用纳米颗粒的广泛研究。本研究主要旨在通过电泳检测比较DNA - C、L的吸光度和稳定性，以研究磁性纳米颗粒的作用。
采用化学共沉淀法合成具有二氧化硅涂层核壳结构的FeO磁性纳米颗粒（MNPs）。这项工作在15分钟内分多次完成，即20次，制备出尺寸为20 nm、形态良好且规则的纳米颗粒，并通过能谱分析（EDX分析）等工具对其结构进行分析。接下来，在单独的容器中，将20 mg磁性纳米颗粒在25 μg/ml的每种混合DNA（环状、线性）溶液的非均相溶液中用二氧化硅包覆。最后，15分钟后，DNA吸附在纳米颗粒表面。通过分光光度法（紫外可见分光光度法，准确率达99%，并用从其纯溶液中分离DNA所需的标准Tris.HCl缓冲液进行优化[未吸附的DNA]）和电泳法测定这种吸附量。
结果表明，纳米颗粒表面DNA - C或L的吸附和扩散率分别为95%和85%（即DNA - C的吸光度>DNA - L），在MNPs上的去除率>99%。因此，经审查，我们发现纳米颗粒与生物分子之间形成了静电键合，能谱分析（EDX分析）结果证实了本研究。
在本项目中，合成了含磁性纳米颗粒的纳米复合材料，并通过相关分析确定了其结构。然后将其用于稳定生物分子，在两种类型的DNA（线性和环状）吸附中分别产生了85%和95%的竞争结果。
