Binandeh Mansour, Rostamnia Sadegh, Karimi Farrokh
Faculty of Science, Department of Chemistry, University of Maragheh, Iran.
Faculty of Science, Department of Biotechnology and Environmental, University of Maragheh, Iran.
Biochem Biophys Rep. 2021 Oct 23;28:101159. doi: 10.1016/j.bbrep.2021.101159. eCollection 2021 Dec.
The aim of this project is to investigate the method of using a common buffer to determine the degree of stabilization and secretion of two drug molecules that have been analyzed in vitro. First, magnetic nanoparticles were synthesized and their structure was identified by instruments such as XPS (X-ray photoelectron spectroscopy) and FT-IR (Fourier transform infrared spectroscopy). The main purpose of this study was to investigate the stabilization and release of methotrexate on the surface of magnetic nanoparticles. The two temperatures were 37 and 25°, respectively. After reaction with the biomolecules, the adsorption rate for both drug molecules was about 60-80. PBS buffer was also used for diffusion of biomolecules and the results were analyzed by spectrophotometer analysis. With these results, the adsorption of cysteine and MTX was more than 60% and its release rate in MNPS-IHSPN was up to 90%, which means that high-strength stabilization and release by magnetic nanoparticles under external magnetic field and in vitro confirmed. The result of this project for the exchange of drugs by the surface of magnetic nanoparticles to repair damaged cells in the body of living organisms can be generalized.
本项目的目的是研究使用一种常见缓冲液来确定两种已在体外分析的药物分子的稳定程度和分泌情况的方法。首先，合成了磁性纳米颗粒，并通过XPS（X射线光电子能谱）和FT-IR（傅里叶变换红外光谱）等仪器对其结构进行了鉴定。本研究的主要目的是研究甲氨蝶呤在磁性纳米颗粒表面的稳定性和释放情况。这两个温度分别为37°和25°。与生物分子反应后，两种药物分子的吸附率约为60-80。还使用PBS缓冲液进行生物分子的扩散，并通过分光光度计分析对结果进行分析。根据这些结果，半胱氨酸和MTX的吸附率超过60%，其在MNPS-IHSPN中的释放率高达90%，这意味着磁性纳米颗粒在外部磁场下和体外实现了高强度的稳定和释放。本项目关于磁性纳米颗粒表面药物交换以修复活生物体体内受损细胞的结果具有普遍意义。
