Binandeh Mansour, Karimi Farrokh, Rostamnia Sadegh
Faculty of Science, Department of Chemistry, University of Maragheh, Iran.
Faculty of Science, Department of Biotechnology and Enviromental, University of Maragheh, Iran.
Ethiop J Health Sci. 2020 Jul 1;30(4):599-606. doi: 10.4314/ejhs.v30i4.15.
In recent years, extensive studies have been performed on magnetite nanoparticles (MNPs) and their applications, which have shown the current project to be one of the major applications by laboratory results.
The nanoparticles synthesized in this project were deposited by the co-precipitation method, which structure was identified by analyzers such as SEM, FT-IR, and EDX. The aim of this project is the adsorption and fixation of biomolecule (BSA (bovine serum albumin) protein on the surface of magnetic nanoparticles.
The adsorption results by electrophoresis and spectrophotometric analyzers showed an absorption rate above 55% ie; 55% of the protein is fixed on the MNPs nanoparticles. This absorption is due to the high level of functionality of magnetic nanoparticles for adsorption of protein. The results of the EDX analysis also show the possible electrostatic bonding between the nanoparticles and the protein, this is derived from -OH with -NH groups of the nanobiocompound (MNPs /protein). After bonding, the two are easily separated.
In this project, the FeO nanoparticles was synthesized and identified by SEM, FT-IR, and EDX analyzers and finally reacted with the BSA protein (for the absorption of protein on MNPs) under experimental conditions at a standard temperature of 25° C. The results showed that about 55% of the protein was fixed on magnetic nanoparticles.
近年来，对磁铁矿纳米颗粒（MNPs）及其应用进行了广泛研究，实验室结果表明当前项目是其主要应用之一。
本项目合成的纳米颗粒采用共沉淀法沉积，其结构通过扫描电子显微镜（SEM）、傅里叶变换红外光谱仪（FT-IR）和能量色散X射线光谱仪（EDX）等分析仪进行鉴定。本项目的目的是将生物分子（牛血清白蛋白（BSA）蛋白）吸附并固定在磁性纳米颗粒表面。
电泳和分光光度分析仪的吸附结果显示吸附率高于55%，即55%的蛋白质固定在MNPs纳米颗粒上。这种吸附归因于磁性纳米颗粒对蛋白质的高吸附功能水平。EDX分析结果还表明纳米颗粒与蛋白质之间可能存在静电结合，这源于纳米生物复合物（MNPs/蛋白质）的-OH与-NH基团。结合后，两者易于分离。
在本项目中，合成了FeO纳米颗粒，并用SEM、FT-IR和EDX分析仪进行了鉴定，最后在25℃的标准温度实验条件下与BSA蛋白反应（用于蛋白质在MNPs上的吸附）。结果表明约55%的蛋白质固定在磁性纳米颗粒上。
