Li Rongjie, Wang Qiaoye, Qiu Yufen, Meng Youshi, Wei Lei, Wang Hao, Mo Ruikang, Zou Donghua, Liu Chunbin
Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, China.
Department of Internal Medicine, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, China.
Front Psychiatry. 2021 Feb 23;12:628361. doi: 10.3389/fpsyt.2021.628361. eCollection 2021.
Competing endogenous RNA (ceRNA) and autophagy were related to neurological diseases. But the relationship among ceRNA, autophagy and Schizophrenia (SZ) was not clear. In this study, we obtained gene expression profile of SZ patients (GSE38484, GSE54578, and GSE16930) from Gene Expression Omnibus (GEO) database. Then we screened the autophagy-related differentially expressed lncRNA, miRNA, and mRNA (DElncRNA, DEmiRNA, and DEmRNA) combined with Gene database from The National Center for Biotechnology Information (NCBI). In addition, we performed enrichment analysis. The result showed that biological processes (BPs) mainly were associated with cellular responses to oxygen concentration. The enriched pathways mainly included ErbB, AMPK, mTOR signaling pathway and cell cycle. Furthermore, we constructed autophagy-related ceRNA network based on the TargetScan database. Moreover, we explored the diagnostic efficiency of lncRNA, miRNA and mRNA in ceRNA, through gene set variation analysis (GSVA). The result showed that the diagnostic efficiency was robust, especially miRNA (AUC = 0.884). The miRNA included hsa-miR-423-5p, hsa-miR-4532, hsa-miR-593-3p, hsa-miR-618, hsa-miR-4723-3p, hsa-miR-4640-3p, hsa-miR-296-5p, and hsa-miR-3943. The result of this study may be helpful for deepening the pathophysiology of SZ. In addition, our finding may provide a guideline for the clinical diagnosis of SZ.
竞争性内源性RNA（ceRNA）与自噬均与神经疾病相关。但ceRNA、自噬与精神分裂症（SZ）之间的关系尚不清楚。在本研究中，我们从基因表达综合数据库（GEO）获取了SZ患者的基因表达谱（GSE38484、GSE54578和GSE16930）。然后我们结合美国国立生物技术信息中心（NCBI）的基因数据库筛选出自噬相关的差异表达长链非编码RNA、微小RNA和信使RNA（DElncRNA、DEmiRNA和DEmRNA）。此外，我们进行了富集分析。结果显示，生物学过程（BP）主要与细胞对氧浓度的反应相关。富集的通路主要包括表皮生长因子受体（ErbB）、腺苷酸活化蛋白激酶（AMPK）、雷帕霉素靶蛋白（mTOR）信号通路和细胞周期。此外，我们基于TargetScan数据库构建了自噬相关的ceRNA网络。而且，我们通过基因集变异分析（GSVA）探索了ceRNA中lncRNA、miRNA和mRNA的诊断效率。结果显示诊断效率可靠，尤其是微小RNA（曲线下面积[AUC]=0.884）。这些微小RNA包括hsa-miR-423-5p、hsa-miR-4532、hsa-miR-593-3p、hsa-miR-618、hsa-miR-4723-3p、hsa-miR-4640-3p、hsa-miR-296-5p和hsa-miR-3943。本研究结果可能有助于深入了解SZ的病理生理学。此外，我们的发现可能为SZ的临床诊断提供指导。
