从海胆胚胎中鉴定出γ样DNA聚合酶。

Identification of gamma-like DNA polymerase from sea urchin embryos.

作者信息

Habara A, Nagano H, Mano Y

出版信息

Biochim Biophys Acta. 1979 Jan 26;561(1):17-28. doi: 10.1016/0005-2787(79)90486-6.

Abstract

A gamma-like DNA polymerase devoid of DNA polymerase-alpha and -beta activities was prepared from the nuclear fraction of blastulae of the sea urchin, Hemicentrotus pulcherrimus. The enzyme sedimented at the position of an approximate sedimentation coefficient of 3.3 S under high salt conditions by sucrose gradient centrifugation. An isoelectric point was determined to be pH 5.8. The enzyme activity was sensitive to sulfhydryl blocking reagents. Poly(rA) . oligo(dT)12--18 followed by poly(dA) . oligo(dT)12--18 was effectively utilized as a template-primer. From the above results, this polymerase seems to resemble the vertebrate DNA polymerase-gamma.

摘要

从海胆（光棘球海胆）囊胚的核部分制备了一种缺乏DNA聚合酶α和β活性的γ样DNA聚合酶。在高盐条件下，通过蔗糖梯度离心，该酶在沉降系数约为3.3 S的位置沉降。测定其等电点为pH 5.8。该酶活性对巯基封闭试剂敏感。聚（rA）·寡聚（dT）12 - 18接着聚（dA）·寡聚（dT）12 - 18被有效地用作模板引物。根据上述结果，这种聚合酶似乎类似于脊椎动物的DNA聚合酶γ。