加利福尼亚海胆中的DNA聚合酶α和β。
DNA polymerase alpha and beta in the California urchin.
作者信息
Racine F M, Morris P W
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3945-57. doi: 10.1093/nar/5.10.3945.
DNA polymerase alpha and beta were identified in the urchin, Strongylocentrotus purpuratus. The DNA polymerase beta sedimented at 3.4 S, constituted 5% of total DNA polymerase activity, and was resistant to N-ethylmaleimide and high ionic strength. The polymerase alpha sedimented at 6--8 S, was inhibited by N-ethylmalemide or 0.1 M (NH4)2SO4, and was dependent upon glycerol for preservation of activity. Both the polymerases alpha and beta were nuclear associated in embryos. The DNA polymerase alpha was markedly heterogeneous on DEAE-Sephadex ion exchange and showed three modal polymerase species. These polymerase alpha species were indistinguishable by template activity assays but the DNA polymerase associated ribonucleotidyl transferase (Biochemistry 75 : 3106-3113, 1976) was found predominantly with only one of the DNA polymerase alpha species.
在紫海胆（Strongylocentrotus purpuratus）中鉴定出了DNA聚合酶α和β。DNA聚合酶β在3.4 S处沉降，占总DNA聚合酶活性的5%，并且对N-乙基马来酰胺和高离子强度具有抗性。聚合酶α在6 - 8 S处沉降，受到N-乙基马来酰胺或0.1 M硫酸铵的抑制，并且其活性的保存依赖于甘油。聚合酶α和β在胚胎中均与细胞核相关。DNA聚合酶α在DEAE-葡聚糖离子交换柱上表现出明显的异质性，并显示出三种模式聚合酶种类。通过模板活性测定，这些聚合酶α种类无法区分，但发现DNA聚合酶相关的核糖核苷酸转移酶（《生物化学》75 : 3106 - 3113, 1976）主要仅与其中一种DNA聚合酶α种类相关。
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