过氧乙酸消毒剂（III 型）对胃肠镜消毒效果的研究。

Study on the Efficacy of Peracetic Acid Disinfectant (Type III) on Gastrointestinal Endoscopy Disinfection.

机构信息

Department of Gastroenterology, The Second Hospital of Shandong University, Jinan city, Shandong Province, Peoples Republic of China.

出版信息

Surg Laparosc Endosc Percutan Tech. 2021 Mar 12;31(4):395-398. doi: 10.1097/SLE.0000000000000921.

DOI:10.1097/SLE.0000000000000921

PMID:33710104

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8360655/

Abstract

PURPOSE

The purpose of this study was to evaluate the disinfection efficacy of peracetic acid disinfectant (Type III) on gastrointestinal endoscopy.

METHODS

Endoscopes were disinfected, respectively, by 2% glutaraldehyde and peracetic acid disinfectant (Type III) according to the procedures stipulated by the 2016 version of "Regulation for cleaning and disinfection technique of flexible endoscope," then samples were collected through biopsy channel at the specified steps. The bacterial count and pathogenic bacteria of these samples were detected, and hepatitis B virus surface antigen, hepatitis C virus antibody, and Treponemiapallidum antibody were detected by chemiluminescent microparticle immunoassay in peracetic acid disinfectant (Type III) group. The samples from the peracetic acid disinfectant (Type III) group were collected for 5 days continuously.

RESULTS

In total, 56 gastroscopes and 16 colonoscopes were disinfected by 2% glutaraldehyde (GA Group), 46 gastroscopes, and 15 colonoscopes were disinfected by peracetic acid disinfectant (Type III) (PAA Group). After disinfection, the bacterial count was significantly reduced in the 2 groups (P<0.05). In terms of the qualified rate of gastroscopes and total qualified rate, the PAA Group was better than GA Group [the qualified rate of gastroscopes: 97.83% (45/46) vs. 92.86% (52/56), P>0.05; total qualified rate: 98.36% (60/61) vs. 94.44% (68/72), P>0.05], the qualified rate of colonoscopes in the 2 groups were both 100.00% (15/15, 16/16). After disinfecting by peracetic acid disinfectant (Type III), hepatitis B virus surface antigen, anti-hepatitis C virus, and Treponemiapallidum antibody were negative. In term of colonies number detected for 5 days continuously, there was no significant difference at different collection steps (P>0.05).

CONCLUSIONS

Peracetic acid disinfectant (Type III) can be well applied to clinical with meeting the standard of high-level disinfection for gastrointestinal endoscopy, and after disinfecting by peracetic acid disinfectant (Type III), there was no obvious bacterial residue in the biopsy channel.

摘要

目的

本研究旨在评估过氧乙酸消毒剂（III 型）对胃肠内镜的消毒效果。

方法

根据 2016 年版《软式内镜清洗消毒技术规范》的规定，分别用 2%戊二醛和过氧乙酸消毒剂（III 型）对内镜进行消毒，然后在规定的步骤通过活检通道采集样本。检测这些样本的细菌计数和病原菌，并用化学发光微粒子免疫分析法检测过氧乙酸消毒剂（III 型）组中的乙型肝炎病毒表面抗原、丙型肝炎病毒抗体和梅毒螺旋体抗体。连续 5 天从过氧乙酸消毒剂（III 型）组采集样本。

结果

共对 56 根胃镜和 16 根结肠镜用 2%戊二醛（GA 组）进行消毒，对 46 根胃镜和 15 根结肠镜用过氧乙酸消毒剂（III 型）（PAA 组）进行消毒。消毒后，两组的细菌计数均明显减少（P<0.05）。在胃镜和总合格率方面，PAA 组优于 GA 组[胃镜合格率：97.83%（45/46）比 92.86%（52/56），P>0.05；总合格率：98.36%（60/61）比 94.44%（68/72），P>0.05]，两组结肠镜合格率均为 100.00%（15/15，16/16）。用过氧乙酸消毒剂（III 型）消毒后，乙型肝炎病毒表面抗原、抗丙型肝炎病毒和梅毒螺旋体抗体均为阴性。连续 5 天检测菌落数，不同采集步骤无显著差异（P>0.05）。