Department of Stomatology, The Ninth People's Hospital of Chongqing, Chongqing, 400700, China.
Department of Oral Implantology, Stomatological Hospital of Chongqing Medical University, Chongqing, 401147, China.
Inflammation. 2021 Aug;44(4):1607-1619. doi: 10.1007/s10753-021-01445-8. Epub 2021 Mar 12.
Periodontitis is a common chronic inflammation that often occurs in adults. Circular RNAs (circRNAs) play a vital role in inflammation-related diseases. However, the role and potential basis of hsa_circ_0085289 in periodontitis remain unknown. Periodontal ligament cells (PDLCs) were exposed to lipopolysaccharide (LPS) to mimic periodontitis. The levels of circ_0085289, let-7f-5p, and suppressor of cytokine signaling 6 (SOCS6) were determined using qRT-PCR and western blot. The release of inflammatory cytokines was measured via enzyme-linked immunosorbent assay (ELISA). Cell viability and apoptosis were determined using Cell Counting Kit-8, flow cytometry, Caspase-3 Assay Kit, and western blot assays. The association between let-7f-5p and circ_0085289/SOCS6 was validated via dual-luciferase reporter, RNA pull-down, and RIP assays. Circ_0085289 and SOCS6 levels were reduced, and let-7f-5p level was increased in periodontitis patients and LPS-treated PDLCs. LPS stimulation caused PDLC injury and circ_0085289 downregulation. Moreover, circ_0085289 upregulation or let-7f-5p downregulation diminished LPS-triggered PDLC injury. Besides, circ_0085289 promoted SOCS6 expression by absorbing let-7f-5p. Circ_0085289 alleviated LPS-stimulated PDLC injury via targeting let-7f-5p. Moreover, let-7f-5p targeted SOCS6 to affect LPS-resulted PDLC injury. Circ_0085289 alleviated PDLC injury induced by LPS stimulation via modulating let-7f-5p/SOCS6 axis, suggesting a promising biomarker for periodontitis treatment.
牙周炎是一种常见的慢性炎症，常发生于成年人。环状 RNA（circRNA）在炎症相关疾病中发挥重要作用。然而，hsa_circ_0085289 在牙周炎中的作用及其潜在基础尚不清楚。用脂多糖（LPS）处理牙周韧带细胞（PDLCs）以模拟牙周炎。采用 qRT-PCR 和 Western blot 检测 circ_0085289、let-7f-5p 和细胞因子信号转导抑制因子 6（SOCS6）的水平。通过酶联免疫吸附试验（ELISA）测量炎症细胞因子的释放。采用细胞计数试剂盒-8（CCK-8）、流式细胞术、Caspase-3 测定试剂盒和 Western blot 检测细胞活力和凋亡。通过双荧光素酶报告、RNA 下拉和 RIP 测定验证 let-7f-5p 与 circ_0085289/SOCS6 的关联。牙周炎患者和 LPS 处理的 PDLCs 中 circ_0085289 和 SOCS6 水平降低，let-7f-5p 水平升高。LPS 刺激导致 PDLC 损伤和 circ_0085289 下调。此外，circ_0085289 上调或 let-7f-5p 下调可减轻 LPS 触发的 PDLC 损伤。此外，circ_0085289 通过吸收 let-7f-5p 促进 SOCS6 表达。circ_0085289 通过靶向 let-7f-5p 缓解 LPS 刺激的 PDLC 损伤。此外，let-7f-5p 靶向 SOCS6 影响 LPS 引起的 PDLC 损伤。circ_0085289 通过调节 let-7f-5p/SOCS6 轴缓解 LPS 刺激的 PDLC 损伤，提示其可能成为牙周炎治疗的有前途的生物标志物。
