He Meng-Jun, Zuo Deng-Pan, Zhang Zong-Ying, Wang Ying, Han Cheng-Gui
Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Biology (Basel). 2023 Jun 25;12(7):908. doi: 10.3390/biology12070908.
Viruses in the genus infect a wide range of crop plants and cause severe economic crop losses. BrYV belongs to the genus and is transmitted by . However, the changes in transcriptome and proteome profiles of during viral infection are unclear. Here, RNA-Seq and TMT-based quantitative proteomic analysis were performed to compare the differences between viruliferous and nonviruliferous aphids. In total, 1266 DEGs were identified at the level of transcription with 980 DEGs being upregulated and 286 downregulated in viruliferous aphids. At the protein level, among the 18 DEPs identified, the number of upregulated proteins in viruliferous aphids was twice that of the downregulated DEPs. Enrichment analysis indicated that these DEGs and DEPs were mainly involved in epidermal protein synthesis, phosphorylation, and various metabolic processes. Interestingly, the expressions of a number of cuticle proteins and tubulins were upregulated in viruliferous aphids. Taken together, our study revealed the complex regulatory network between BrYV and its vector from the perspective of omics. These findings should be of great benefit to screening key factors involved in the process of virus circulation in aphids and provide new insights for BrYV prevention via vector control in the field.
该属病毒可感染多种农作物，导致严重的经济作物损失。BrYV属于该属，由[具体传播方式未给出]传播。然而，在病毒感染期间[具体对象未给出]的转录组和蛋白质组图谱变化尚不清楚。在此，进行了RNA测序和基于TMT的定量蛋白质组分析，以比较带毒蚜虫和无毒蚜虫之间的差异。总共在转录水平上鉴定出1266个差异表达基因（DEG），其中980个DEG在带毒蚜虫中上调，286个下调。在蛋白质水平上，在鉴定出的18个差异表达蛋白（DEP）中，带毒蚜虫中上调蛋白的数量是下调DEP的两倍。富集分析表明，这些DEG和DEP主要参与表皮蛋白合成、磷酸化和各种代谢过程。有趣的是，许多表皮蛋白和微管蛋白的表达在带毒蚜虫中上调。综上所述，我们的研究从组学角度揭示了BrYV与其载体[具体载体未给出]之间复杂的调控网络。这些发现对于筛选蚜虫体内病毒传播过程中的关键因素应具有很大的益处，并为通过田间控制载体预防BrYV提供新的见解。
