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基于季铵硅烷（k21）的根管内药物引发生物膜破坏。

Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction.

机构信息

Division of Clinical Dentistry, Schoolof Dentistry, International Medical University Kuala Lumpur, Kuala Lumpur, Malaysia.

Faculty of Biomedical Science, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia.

出版信息

BMC Oral Health. 2021 Mar 12;21(1):116. doi: 10.1186/s12903-021-01470-x.

DOI:10.1186/s12903-021-01470-x
PMID:33711992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7953794/
Abstract

BACKGROUND

Compare antimicrobial efficacy of a quarternary ammonium silane (QAS)/k21 as an intracanal medicament against E. faecalis and C. albicans biofilms formed on root dentin.

METHODOLOGY

Dentin blocks were sterilized and E. faecalis and C. albicans microbial colonies were counted for colony-forming-units against 2%k21, 2%CHX and Ca(OH)2 medicaments. Biofilm colonies after 7 days on dentin were analysed using confocal laser scanning microscopy with live/dead bacterial viability staining. TEM was done to study dentin collagen matrix. Dentin discs from 3rd day and 7th day well plate was used for Raman spectra and observed under fluorescent-microscope. Docking studies were carried out on MMP-2 S binding-domain with k21.

RESULTS

There was reduction of E. faecalis/C. albicans when k21, chlorhexidine and calcium hydroxide were used with highest percentage in 2%k21 treated specimens. 2%k21 showed dense and regular collagen network with intact cross-banding and decreased Raman intensity for 2%k21 on 3rd day. NaOCl + k21 showed least adherence, whereas saline groups showed highest adherence of E. faecalis and C. albicans to root-canal dentin. Alizarin red staining of hDPSCs revealed calcium deposition in all groups with significant difference seen amongst 2%k21 groups. MMP-2 ligand binding was seen accurately indicating possible target sites for k21 intervention.

CONCLUSION

2%k21 can be considered as alternative intracanal medicament.

摘要

背景

比较季铵盐硅烷（QAS）/k21 作为根管内药物对形成于根管牙本质上的粪肠球菌和白色念珠菌生物膜的抗菌效果。

方法

对牙本质块进行消毒，并对 2%k21、2%CHX 和 Ca(OH)2 药物的粪肠球菌和白色念珠菌微生物菌落进行菌落形成单位计数。用活/死细菌活力染色对牙本质上 7 天后的生物膜菌落进行共聚焦激光扫描显微镜分析。用 TEM 研究牙本质胶原基质。使用来自第 3 天和第 7 天的牙本质圆盘进行拉曼光谱分析，并在荧光显微镜下观察。对 MMP-2 S 结合域与 k21 进行对接研究。

结果

当使用 k21、洗必泰（chlorhexidine）和氢氧化钙时，粪肠球菌/白色念珠菌的数量减少，其中 2%k21 处理的标本减少率最高。2%k21 显示出密集且规则的胶原网络，具有完整的交叉带且第 3 天的 2%k21 的拉曼强度降低。NaOCl+k21 显示出最低的粘附性，而生理盐水组显示出粪肠球菌和白色念珠菌对根管牙本质的最高粘附性。所有组的 hDPSCs 茜素红染色均显示钙沉积，其中 2%k21 组之间存在显著差异。MMP-2 配体结合被准确观察到，这表明 k21 干预的可能靶点。

结论

2%k21 可被视为替代根管内药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/7a4dee8c3a3e/12903_2021_1470_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/50c90f9a268c/12903_2021_1470_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/a013e5764c37/12903_2021_1470_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/bfccddebde60/12903_2021_1470_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/6778fbe8a1d1/12903_2021_1470_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/8b11ae196417/12903_2021_1470_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/7a4dee8c3a3e/12903_2021_1470_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/50c90f9a268c/12903_2021_1470_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/a013e5764c37/12903_2021_1470_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/bfccddebde60/12903_2021_1470_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/6778fbe8a1d1/12903_2021_1470_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/8b11ae196417/12903_2021_1470_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc47/7953794/7a4dee8c3a3e/12903_2021_1470_Fig6_HTML.jpg

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