一种改性季铵硅烷制剂作为牙髓腔冲洗剂的性能研究。

Properties of a modified quaternary ammonium silane formulation as a potential root canal irrigant in endodontics.

机构信息

Division of Clinical Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan Kuala Lumpur, Malaysia.

出版信息

Dent Mater. 2020 Dec;36(12):e386-e402. doi: 10.1016/j.dental.2020.09.008. Epub 2020 Oct 1.

DOI:10.1016/j.dental.2020.09.008

PMID:33010944

Abstract

OBJECTIVES

Evaluate a new modified quaternary ammonium silane irrigant solution for its antimicrobial, cytotoxic and mechanical properties of dentine substrate.

METHODS

Root canal preparation was performed using stainless steel K-files™ and F4 size protaper with irrigation protocols of 6% NaOCl + 2% CHX; 3.5% QIS; 2% QIS and sterile saline. Biofilms were prepared using E. faecalis adjusted and allowed to grow for 3 days, treated with irrigants, and allowed to grow for 7 days. AFM was performed and surface free energy calculated. MC3T3 cells were infected with endo irrigant treated E. faecalis biofilms. Raman spectroscopy of biofilms were performed after bacterial re-growth on root dentine and exposed to different irrigation protocols and collagen fibers analysed collagen fibers using TEM. Antimicrobial potency against E. faecalis biofilms and cytoxicity against 3T3 NIH cells were also. Resin penetration and MitoTracker green were also evaluated for sealer penetration and mitochondrial viability. Data were analysed using One-way ANOVA, principal component analysis and post-hoc Fisher's least-significant difference.

RESULTS

Elastic moduli were maintained amongst control (5.5 ± 0.9) and 3.5% QIS (4.4 ± 1.1) specimens with surface free energy higher in QIS specimens. MC3T3 cells showed reduced viability in 6%NaOCl+2%CHX specimens compared to QIS specimens. DNA/purine were expressed in increased intensities in control and 6% NaOCl + 2% CHX specimens with bands around 480-490 cm reduced in QIS specimens. 3.5% QIS specimens showed intact collagen fibrillar network and predominantly dead bacterial cells in confocal microscopy. 3.5% QIS irrigant formed a thin crust-type surface layer with cytoplasmic extensions of 3T3NIH spread over root dentine. Experiments confirmed MitoTracker accumulation in 3.5% treated cells.

SIGNIFICANCE

Novel QIS root canal irrigant achieved optimum antimicrobial protection inside the root canals facilitating a toxic effect against the Enterococcus faecalis biofilm. Root dentine substrates exhibited optimum mechanical properties and there was viability of fibroblastic mitochondria.

摘要

目的

评估一种新型改良的季铵盐硅烷冲洗液在根管内的抗菌、细胞毒性和牙本质基质的机械性能。

方法

使用不锈钢 K 文件和 F4 号扩锉针进行根管预备，并采用 6% NaOCl+2% CHX、3.5% QIS、2% QIS 和无菌生理盐水的冲洗方案。用粪肠球菌调整生物膜并使其生长 3 天，用冲洗液处理后再生长 7 天。用原子力显微镜（AFM）进行检测并计算表面自由能。将感染根管内冲洗液的粪肠球菌生物膜的 MC3T3 细胞接种，在生物膜重新生长后，用不同的冲洗方案对其进行处理，并用拉曼光谱法分析生物膜中的胶原纤维，并用透射电子显微镜分析胶原纤维。还评估了对粪肠球菌生物膜的抗菌效力和对 3T3 NIH 细胞的细胞毒性。还评估了树脂渗透和 MitoTracker 绿在密封剂渗透和线粒体活力方面的作用。使用单向方差分析、主成分分析和事后 Fisher 最小显著差异进行数据分析。

结果

与对照（5.5±0.9）和 3.5% QIS（4.4±1.1）标本相比，弹性模量在 3.5% QIS 标本中保持不变，且表面自由能更高。与 QIS 标本相比，MC3T3 细胞在 6% NaOCl+2% CHX 标本中的活力降低。与对照和 6% NaOCl+2% CHX 标本相比，DNA/嘌呤在 6% NaOCl+2% CHX 标本中的表达强度增加，而 QIS 标本中的 480-490cm 左右的带减少。在共聚焦显微镜下，3.5% QIS 标本显示完整的胶原纤维网络和主要为死细菌细胞。3.5% QIS 冲洗液在根管内形成一层薄的壳状表面层，3T3NIH 的细胞质延伸覆盖在根管牙本质上。实验证实了 MitoTracker 在 3.5%处理细胞中的积累。