一种新型季铵盐硅烷根管冲洗剂的体外研究。

An in vitro study of a novel quaternary ammonium silane endodontic irrigant.

机构信息

Clinical Dentistry, Restorative Division, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, 57000 Bukit Jalil, Wilayah Persekutuan Kuala Lumpur, Malaysia.

出版信息

Dent Mater. 2019 Sep;35(9):1264-1278. doi: 10.1016/j.dental.2019.05.020. Epub 2019 Jun 12.

DOI:10.1016/j.dental.2019.05.020

PMID:31201019

Abstract

OBJECTIVE

To analyze effect of NaOCl+2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate.

METHODS

Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl+2% CHX, 6% NaOCl+2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days.

RESULTS

Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl+2% QAS group. There was reduction of 484cm Raman band and its intensity reached lowest with NaOCl+2% QAS. There was an increase in 1350-1420cm intensity in the NaOCl+2% CHX groups. Gradual decrease in 1639cm and 1609cm Raman signal ratios were seen in the resin-depth region of 17μm>, 14.1μm> and 13.2μm for NaOCl+2% QAS, NaOCl+2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl+2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl+2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl+2% QAS.

SIGNIFICANCE

Favorable antimicrobial and endodontic profile of the NaOCl+2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.

摘要

目的

分析新型含 NaOCl+2%季铵盐（QAS）冲洗液对根管内细菌的作用，并评价其对牙本质基质的抗菌和机械性能。

方法

使用不锈钢 K 锉和 ProTaper 根管预备系统，采用手动和超声冲洗，分别使用 6% NaOCl+2% CHX、6% NaOCl+2% QAS 和生理盐水作为对照。对于使用 Enterococcus faecalis 培养 7 天的共聚焦显微镜、拉曼光谱和 SEM 分析，在处理前后进行。使用 Berkovich 压头评估机械性能的压痕。评估冲洗剂的接触角和表面自由能。在 14 天后通过茜素红检测矿化结节。

结果

对照组生物膜显示密集的绿色菌落。NaOCl+2% QAS 组中，大多数 E. faecalis 细菌存在于生物膜中，发出红色荧光。在 NaOCl+2% QAS 组中，484cm 拉曼带的强度降低，达到最低水平。在 NaOCl+2% CHX 组中，1350-1420cm 强度增加。在 NaOCl+2% QAS、NaOCl+2% CHX 和对照组的树脂深度区域 17μm>、14.1μm>和 13.2μm 中，1639cm 和 1609cm 拉曼信号强度比逐渐降低。所有被封闭的组都显示出完整的密封剂/牙本质界面，只有一些明显的差异。NaOCl+2% QAS 超声组的 0.771 和 83.5% 蠕变压痕距离。NaOCl+2% QAS 组的极性成分比例最高，明显高于其他组。NaOCl+2% QAS 组矿化结节增加。