Hu Yan, Wang Zhe, Xia Fangbo, Yang Wen, Liu Yuan-Cai, Wan Jian-Bo
State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China.
Chin Med. 2021 Mar 12;16(1):26. doi: 10.1186/s13020-021-00435-0.
The Chinese medicinal wine made from herbal medicines became prevalent among Chinese people. The Chinese herbal spirit is composed of several herbal extracts, and has the certain health functions, such as anti-fatigue and immune regulation. The quality evaluation of Chinese herbal spirit is greatly challenged by the enormous and complex components with great structural diversity and wide range of concentration distribution.
An ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS) with multiple reaction monitoring (MRM) method was developed to simultaneously determine forty-three bioactive components in the Chinese herbal spirits produced by year 2014 and 2018.
Quantitative results showed that 11 components, i.e.., puerarin (5), purpureaside C (7), daidzin (8), echinacoside (9), acteoside (15), epimedin B (22), epimedin C (23), icariin (24), eugenol (27), chikusetsusaponin iva (30) and Z-ligustilide (40), significantly decreased along with the increasing years of storage, while 5 compounds, i.e.., geniposidic acid (1), protocatechuic acid (2), crustecdysone (14), daidzein (18) and icariside I (35), were basically stable in all samples across the years.
The established method allowing to simultaneously determined 43 components with wide structural diversity and trace amounts will facilitate the quality control research of Chinese herbal spirits.
由草药制成的中国药酒在中国人中很流行。中国草本酒由几种草药提取物组成，具有一定的保健功能，如抗疲劳和免疫调节。中国草本酒的质量评价受到其巨大而复杂的成分的极大挑战，这些成分结构多样，浓度分布范围广。
建立了一种超高效液相色谱-三重四极杆质谱联用（UHPLC-QQQ-MS/MS）的多反应监测（MRM）方法，用于同时测定2014年和2018年生产的中国草本酒中的43种生物活性成分。
定量结果表明，11种成分，即葛根素（5）、紫菀苷C（7）、大豆苷（8）、松果菊苷（9）、毛蕊花糖苷（15）、朝藿定B（22）、朝藿定C（23）、淫羊藿苷（24）、丁香酚（27）、竹节人参皂苷IVa（30）和Z-藁本内酯（40），随着储存年限的增加而显著降低，而5种化合物，即京尼平苷酸（1）、原儿茶酸（2）、蜕皮甾酮（14）、大豆黄酮（18）和淫羊藿次苷I（35），在所有年份的样品中基本稳定。
所建立的方法能够同时测定结构多样、含量微量的43种成分，将有助于中国草本酒的质量控制研究。
