Qian Dongyang, Zhou Hui, Fan Pan, Yu Tao, Patel Anish, O'Brien Morgan, Wang Zhe, Lu Shiguang, Tong Guoqiang, Shan Yimin, Wang Lei, Gao Yuan, Xiong Yuan, Zhang Lily, Wang Xin, Liu Yuancai, Zhou Shuanhu
Department of Orthopedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, United States.
Department of Orthopedics, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Front Pharmacol. 2021 Dec 15;12:754088. doi: 10.3389/fphar.2021.754088. eCollection 2021.
Traditional Chinese medicine (TCM) has been practiced in the treatment of bone diseases and alcoholism. Chronic excessive alcohol use results in alcohol-induced bone diseases, including osteopenia and osteoporosis, which increases fracture risk, deficient bone repair, and osteonecrosis. This preclinical study investigated the therapeutic effects of TCM herbal extracts in animal models of chronic excessive alcohol consumption-induced osteopenia. TCM herbal extracts (Jing extracts) were prepared from nine Chinese herbal medicines, a combinative herbal formula for antifatigue and immune regulation, including , , , , , , , , and . In this study, Balb/c male mice were orally administrated alcohol (3.2 g/kg/day) with/without TCM herbal extracts (0.125 g/kg, 0.25 g/kg, or 0.5 g/kg) by gavage. Our results showed that after 50 days of oral administration, TCM herbal extracts prevented alcohol-induced osteopenia demonstrated by μ-CT bone morphological analysis in young adults and middle-aged/old Balb/c male mice. Biochemical analysis demonstrated that chronic alcohol consumption inhibits bone formation and has a neutral impact on bone resorption, suggesting that TCM herbal extracts (Jing extracts) mitigate the alcohol-induced abnormal bone metabolism in middle-aged/old male mice. Protocatechuic acid, a natural phenolic acid in Jing extracts, mitigates alcohol-induced decline of alkaline phosphatase (ALP) gene expression in the bone marrow of Balb/c male mice and ALP activity in pre-osteoblast MC3T3-E1 cells. Our study suggests that TCM herbal extracts prevent chronic excessive alcohol consumption-induced osteopenia in male mice, implying that traditional medicinal plants have the therapeutic potential of preventing alcohol-induced bone diseases.
传统中医（TCM）已被应用于骨疾病和酒精成瘾的治疗。长期过量饮酒会导致酒精性骨疾病，包括骨质减少和骨质疏松，这会增加骨折风险、影响骨修复以及导致骨坏死。这项临床前研究调查了中药提取物对慢性过量饮酒诱导的骨质减少动物模型的治疗效果。中药提取物（荆提取物）由九种中药制备而成，这是一种用于抗疲劳和免疫调节的复方草药配方，包括[此处原文缺失九种中药具体名称]。在本研究中，通过灌胃给Balb/c雄性小鼠口服酒精（3.2克/千克/天），同时给予/不给予中药提取物（0.125克/千克、0.25克/千克或0.5克/千克）。我们的结果表明，口服给药50天后，中药提取物预防了酒精诱导的骨质减少，这在年轻成年和中年/老年Balb/c雄性小鼠的μ-CT骨形态分析中得到证实。生化分析表明，长期饮酒会抑制骨形成，并对骨吸收有中性影响，这表明中药提取物（荆提取物）减轻了中年/老年雄性小鼠中酒精诱导的异常骨代谢。原儿茶酸是荆提取物中的一种天然酚酸，它减轻了酒精诱导的Balb/c雄性小鼠骨髓中碱性磷酸酶（ALP）基因表达的下降以及前成骨细胞MC3T3-E1细胞中ALP活性的下降。我们的研究表明，中药提取物可预防雄性小鼠慢性过量饮酒诱导的骨质减少，这意味着传统药用植物具有预防酒精性骨疾病的治疗潜力。
