Printivo Group AD, Sofia, Bulgaria; Medical University of Sofia, 2 Zdrave Street, 1431 Sofia, Bulgaria.
Medical University of Pleven, Pleven, Bulgaria.
Carbohydr Polym. 2021 May 15;260:117793. doi: 10.1016/j.carbpol.2021.117793. Epub 2021 Feb 13.
The present study demonstrates the extrusion printing of highly viscous and thixotropic hydroxyethylcellulose-based bioinks blended with various concentrations of sodium alginate (SA) and embedded with HeLa cells. The cell viability is shown to be inversely proportional to the relative SA content and can be as high as 81.5 % following one day of incubation. Furthermore, the biocompatibility of the hydrogel matrix supports cell proliferation resulting in an order of magnitude larger number of cells after a 7-day incubation. The cell viability is negatively affected mostly by the extrusion printing itself with some cell death occurring during their embedding in the hydrogels. After embedding the HeLa cells in the blends containing 1 and 2.5 % SA, the cell viability is not significantly affected by the residence time of up to 90 min before the bioink extrusion. The printed constructs can be utilized as a cervical tumor model.
本研究展示了高度粘稠和触变羟乙基纤维素基生物墨水的挤出打印，该生物墨水与不同浓度的海藻酸钠（SA）混合，并嵌入了 HeLa 细胞。结果表明，细胞活力与相对 SA 含量成反比，孵育一天后可高达 81.5%。此外，水凝胶基质的生物相容性支持细胞增殖，孵育 7 天后细胞数量增加了一个数量级。细胞活力主要受到挤出打印本身的负面影响，在将 HeLa 细胞嵌入水凝胶的过程中会发生一些细胞死亡。将 HeLa 细胞嵌入含有 1%和 2.5%SA 的混合物中后，在生物墨水挤出之前，其在 90 分钟内的停留时间不会显著影响细胞活力。打印的结构可以用作宫颈肿瘤模型。
