US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Commun Biol. 2021 Mar 12;4(1):333. doi: 10.1038/s42003-021-01859-y.
Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.
微藻能够高效地将阳光转化为脂质和碳水化合物，为能源和化学品生产提供了基于生物的替代方案。提高藻类的生产力和对非生物胁迫的抗性需要通过功能基因组学进行系统水平的表征。在这里，我们对一种耐盐微藻 Scenedesmus sp. NREL 46B-D3 进行了表征，该微藻在接近 25°C 的温度下达到峰值生长，每天达到 30g/m，并且在耐盐菌株中报道了迄今为止最高的细胞分裂后生物量积累能力。功能基因组学分析表明，参与脂质生产、离子通道和转运蛋白的基因得到了扩展和表达。暴露于温度胁迫会改变脂肪酸代谢并增加氨基酸的合成。共表达分析表明，在冷胁迫下，许多脂肪酸生物合成基因与特定的转录因子一起过表达。这些以及其他参与对温度胁迫的代谢和调节反应的基因，可以进一步探索用于菌株改良。
