Department of Chemical and Materials Engineering, Tunghai University, Taiwan, ROC.
Department of Chemical and Materials Engineering, Tunghai University, Taiwan, ROC.
Bioresour Technol. 2015 May;184:148-152. doi: 10.1016/j.biortech.2014.09.113. Epub 2014 Sep 28.
In this co-culture of oleaginous yeast-Rhodotorula glutinis and microalgae-Scenedesmus obliquus, microalgae potentially acts as an oxygen generator for the growth of aerobic yeast while the yeast mutually provides CO2 to the microalgae as both carry out the production of lipids. To explore the synergistic effects of co-cultivation on the cells growth and total lipids accumulation, several co-culture process parameters including the carbon source concentration, temperature and dissolved oxygen level would be firstly investigated in the flask trials. The results of co-culture in a 5L photobioreactor revealed that about 40-50% of biomass increased and 60-70% of total lipid increased was observed as compared to the single culture batches. Besides the synergistic effects of gas utilization, the providing of trace elements to each other after the natural cells lysis was believed to be another benefit to the growth of the overall co-culture system.
在产油酵母——粘红酵母和微藻——斜生栅藻的共培养中,微藻可以作为好氧酵母生长的氧气发生器,而酵母则相互为微藻提供二氧化碳,因为两者都在进行脂质生产。为了探索共培养对细胞生长和总脂积累的协同作用,首先将在摇瓶试验中研究几种共培养过程参数,包括碳源浓度、温度和溶解氧水平。与单一培养批次相比,在 5L 光生物反应器中的共培养结果表明,生物量增加了约 40-50%,总脂增加了 60-70%。除了气体利用的协同作用外,自然细胞裂解后相互提供微量元素被认为是对整个共培养系统生长的另一个益处。