Wu Yu, Liu Huimin, Bing Jie, Zhang Genfa
Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.
Front Plant Sci. 2022 Oct 21;13:1035750. doi: 10.3389/fpls.2022.1035750. eCollection 2022.
Seed germination is critical for plant survival and agricultural production and is affected by many cues, including internal factors and external environmental conditions. As a key enzyme in glycolysis, enolase 2 (ENO2) also plays a vital role in plant growth and abiotic stress responses. In our research, we found that the seed germination rate was lower in the mutation ( ) than in the wild type (WT) under salt stress in , while there was no significant difference under normal conditions. However, the mechanisms by which regulates seed germination under salt stress remain limited. In the current study, transcriptome and proteome analyses were used to compare and the WT under normal and salt stress conditions at the germination stage. There were 417 and 4442 differentially expressed genes (DEGs) identified by transcriptome, and 302 and 1929 differentially expressed proteins (DEPs) qualified by proteome under normal and salt stress conditions, respectively. The combined analysis found abundant DEGs and DEPs related to stresses and hydrogen peroxide removal were highly down-regulated in . In addition, several DEGs and DEPs encoding phytohormone transduction pathways were identified, and the DEGs and DEPs related to ABA signaling were relatively greatly up-regulated in . Moreover, we constructed an interactive network and further identified GAPA1 and GAPB that could interact with AtENO2, which may explain the function of AtENO2 under salt stress during seed germination. Together, our results reveal that under salt stress, mainly affects the expression of genes and proteins related to the phytohormone signal transduction pathways, stress response factors, and reactive oxygen species (ROS), and then affects seed germination. Our study lays the foundation for further exploration of the molecular function of under salt stress at the seed germination stage in .
种子萌发对于植物存活和农业生产至关重要，且受到许多因素的影响，包括内部因素和外部环境条件。作为糖酵解中的关键酶，烯醇化酶2（ENO2）在植物生长和非生物胁迫响应中也起着至关重要的作用。在我们的研究中，我们发现，在盐胁迫下，突变体（ ）的种子萌发率低于野生型（WT），而在正常条件下两者无显著差异。然而， 在盐胁迫下调节种子萌发的机制仍然有限。在当前研究中，利用转录组和蛋白质组分析来比较 和WT在萌发阶段正常和盐胁迫条件下的情况。在正常和盐胁迫条件下，转录组分别鉴定出417个和4442个差异表达基因（DEG），蛋白质组分别鉴定出302个和1929个差异表达蛋白质（DEP）。联合分析发现，与胁迫和过氧化氢清除相关的大量DEG和DEP在 中高度下调。此外，鉴定出了几个编码植物激素转导途径的DEG和DEP，与脱落酸信号相关的DEG和DEP在 中上调幅度相对较大。此外，我们构建了一个相互作用网络，并进一步鉴定出了可与AtENO2相互作用的GAPA1和GAPB，这可能解释了AtENO2在种子萌发期间盐胁迫下的功能。总之，我们的结果表明，在盐胁迫下， 主要影响与植物激素信号转导途径、胁迫响应因子和活性氧（ROS）相关的基因和蛋白质的表达，进而影响种子萌发。我们的研究为进一步探索 在 种子萌发阶段盐胁迫下的分子功能奠定了基础。
