Department of Sport Sciences, Shahrekord University, Shahrekord, Iran.
Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
Exp Gerontol. 2021 Jul 1;149:111305. doi: 10.1016/j.exger.2021.111305. Epub 2021 Mar 11.
The crosstalk between skeletal muscles and other tissues such as bones is typically established via the secretion of myokines and myomiRs induced by exercise training (ET). The present study aimed at evaluating the relationship between changes made by different ET modes and intensities in myomiRs, osteomiRs, and other myogenic and osteogenic biomarkers in old male Wistar rats. To this end, a total number of 50 old (23 months of age) male Wistar rats were randomly assigned to four experimental groups, namely, moderate-intensity endurance training (MIET), high-intensity endurance training (HIET), moderate-intensity resistance training (MIRT), high-intensity resistance training (HIRT), and control (CON), each one comprised of 10 subjects. The study findings revealed positive correlations between myomiRs (i.e., miR-1) and myomiR-204a (r = 0.725; p = 0.042), myomiR-1, and runt-related transcription factor 2 (RUNX2) osteogenic marker (r = 0.869; p = 0.025) in the HIET group, myomiR-206 and peroxisome proliferator-activated receptor gamma (PPARγ) (r = 0.908; p = 0.012) in the MIRT group, myomiR-133a and osteomiR-133a (r = 0.971; p = 0.005) in the MIET group, myomiR-133a and osteomiR-204a in the MIRT group (r = 0.971; p = 0.004), and myomiR-133a and RUNX2 gene expression in the HIET group (r = 0.861; p = 0.027). It was concluded that myomiRs involved in myoblast-osteoblast differentiation might not alone regulate the myogenic and osteogenic targets in response to different modes and intensities of ET treatments.
骨骼肌与骨骼等其他组织之间的串扰通常是通过运动训练（ET）诱导的肌因子和肌微小 RNA（miR）的分泌来建立的。本研究旨在评估不同 ET 模式和强度对老年雄性 Wistar 大鼠的肌微小 RNA（miR）、骨微小 RNA（osteomiR）和其他成肌和成骨生物标志物的影响。为此，将 50 只老年（23 个月龄）雄性 Wistar 大鼠随机分为 4 个实验组，即中等强度耐力训练（MIET）、高强度耐力训练（HIET）、中等强度抗阻训练（MIRT）、高强度抗阻训练（HIRT）和对照组（CON），每组 10 只。研究结果表明，在 HIET 组中，miR-1 与肌微小 RNA-204a（r=0.725；p=0.042）、miR-1 与成骨转录因子 2（RUNX2）（r=0.869；p=0.025）之间存在正相关，在 MIRT 组中，miR-206 与过氧化物酶体增殖物激活受体γ（PPARγ）（r=0.908；p=0.012）之间存在正相关，在 MIET 组中，miR-133a 与骨微小 RNA-133a（r=0.971；p=0.005）之间存在正相关，在 MIRT 组中，miR-133a 与骨微小 RNA-204a（r=0.971；p=0.004）之间存在正相关，在 HIET 组中，miR-133a 与 RUNX2 基因表达（r=0.861；p=0.027）之间存在正相关。结论是，参与成肌细胞-成骨细胞分化的肌微小 RNA 可能不会单独调节不同模式和强度的 ET 治疗对成肌和成骨靶标的影响。
