National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Food Chem. 2021 Aug 15;353:129452. doi: 10.1016/j.foodchem.2021.129452. Epub 2021 Mar 3.
This study aimed to investigate the protective effects of a < 3 kDa Tricholoma matsutake Singer peptide (TMWP) on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. The results showed that TMWP significantly upregulated superoxide dismutase (SOD) activity and reduced reactive oxygen species (ROS) generation in RAW264.7 macrophages. Western blotting and immunofluorescence analysis indicated that TMWP inhibited the activation of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways, thereby reducing the secretion of IL-1β and IL-6 and the expression of TNF-α, COX-2, and iNOS. Additionally, TMWP improved mitochondrial respiration in LPS-stressed macrophages, counteracting the harmful effects of LPS treatment on mitochondrial function. Three peptides (SDIKHFPF, SDLKHFPF, and WFNNAGP) with the highest predicted scores for potential anti-inflammatory activity were identified using nano-HPLC-MS/MS. These data indicated that T. matsutake peptides could be an attractive natural ingredient for developing novel functional foods.
本研究旨在探讨<3 kDa 松茸肽（TMWP）对脂多糖（LPS）诱导的 RAW264.7 巨噬细胞炎症的保护作用。结果表明，TMWP 可显著上调 RAW264.7 巨噬细胞中超氧化物歧化酶（SOD）活性，降低活性氧（ROS）的产生。Western blot 和免疫荧光分析表明，TMWP 抑制丝裂原活化蛋白激酶（MAPK）和核因子κB（NF-κB）通路的激活，从而减少 IL-1β和 IL-6 的分泌以及 TNF-α、COX-2 和 iNOS 的表达。此外，TMWP 改善了 LPS 应激巨噬细胞中的线粒体呼吸，抵消了 LPS 处理对线粒体功能的有害影响。使用纳升 HPLC-MS/MS 鉴定出三个具有最高预测抗炎活性评分的肽（SDIKHFPF、SDLKHFPF 和 WFNNAGP）。这些数据表明，松茸肽可能是开发新型功能性食品的有吸引力的天然成分。
