Li Mengqi, Ge Qi, Du Hanting, Lin Songyi
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods. 2021 Nov 3;10(11):2680. doi: 10.3390/foods10112680.
is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of . Our aim was to evaluate the protective effects and molecular mechanisms of two -derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage.
是一种可食用真菌，含有多种生物活性物质，其中一些具有免疫刺激特性。目前，关于其功能成分的了解有限。我们的目的是评估两种源自的肽SDLKHFPF和SDIKHFPF对脂多糖（LPS）诱导的RAW264.7巨噬细胞线粒体功能障碍和炎症的保护作用及分子机制。肽显著改善了炎性细胞因子的产生，并抑制了COX-2、iNOS、IKKβ、p-IκB-α和p-NF-κB的表达。免疫荧光分析证实了肽对NF-κB/p65核转位的抑制作用。此外，肽处理可防止活性氧的积累，增加Bcl-2/Bax比值，逆转线粒体膜电位的丧失，并挽救细胞能量代谢异常。这些发现表明，肽可有效抑制NF-κB/COX-2的激活，并可能对LPS诱导的细胞损伤具有全面的保护作用。
