Kikuchi Wataru, Ichihara Kiyoshi, Mori Kazuo, Shimizu Yoshihisa
Research and Development Headquarters, Nitto Boseki Co., Ltd, Koriyama, Japan.
Department of Clinical Laboratory Sciences, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Ann Clin Biochem. 2021 Jul;58(4):358-367. doi: 10.1177/00045632211003941. Epub 2021 Apr 7.
Tartrate-resistant acid phosphatase 5b (TRACP5b) is a bone resorption marker that is mainly used in clinical management of osteoporosis. For proper interpretations of test results for serum TRACP5b, we explored their biological sources of variation, esp. age-related changes, and associations with other bone-related markers in healthy Japanese adults.
During the 2009 East-Southeast Asian multicentre study for determination of reference intervals, 72 major laboratory tests were measured by centralized assays in 3541 well-defined healthy volunteers. The current study included 1980 test results in Japanese subjects for five bone-related markers: TRACP5b, bone alkaline phosphatase, intact parathyroid hormone, calcium and inorganic phosphate. Information on sources of variation, including body mass index, smoking habits and ABO-blood group, were obtained from a health status questionnaire.
Gender-specific profiles of age-related changes were observed for each parameter. Increased values starting from 40 years of age in females were most prominent for TRACP5b, followed by bone alkaline phosphatase and inorganic phosphate. TRACP5b in males decreased with body mass index, bone alkaline phosphatase and TRACP5b were higher in blood type-O subjects, especially in males. TRACPT5b was closely correlated with bone alkaline phosphatase, and moderately correlated with adjusted calcium and inorganic phosphate, especially in females aged ≥45 years. Reference intervals for each analyte were determined parametrically based on gender and age.
This study elucidated sources of variation of TRACP5b and related bone markers in healthy Japanese subjects and demonstrated a specific age profile for each marker. These results are of relevance for better clinical usage and interpretations of serum levels of bone markers.
抗酒石酸酸性磷酸酶5b（TRACP5b）是一种骨吸收标志物，主要用于骨质疏松症的临床管理。为了正确解读血清TRACP5b的检测结果，我们探讨了其生物学变异来源，特别是与年龄相关的变化，以及与健康日本成年人其他骨相关标志物的关联。
在2009年东亚 - 东南亚多中心参考区间测定研究中，对3541名明确界定的健康志愿者进行集中检测，测量了72项主要实验室指标。本研究纳入了1980例日本受试者的5种骨相关标志物的检测结果：TRACP5b、骨碱性磷酸酶、完整甲状旁腺激素、钙和无机磷。通过健康状况问卷获取包括体重指数、吸烟习惯和ABO血型等变异来源的信息。
观察到每个参数与年龄相关变化的性别特异性特征。女性从40岁开始数值升高，这在TRACP5b中最为明显，其次是骨碱性磷酸酶和无机磷。男性的TRACP5b随体重指数降低，O型血受试者的骨碱性磷酸酶和TRACP5b较高，尤其是男性。TRACPT5b与骨碱性磷酸酶密切相关，与校正后的钙和无机磷中度相关，尤其是在年龄≥45岁的女性中。基于性别和年龄参数确定了每种分析物的参考区间。
本研究阐明了健康日本受试者中TRACP5b及相关骨标志物的变异来源，并展示了每种标志物的特定年龄特征。这些结果对于更好地临床应用和解读骨标志物血清水平具有重要意义。
