Yu Yihan, Hu Qiuhui, Liu Jianhui, Su Anxiang, Xu Hui, Li Xiuting, Huang Qingrong, Zhou Jinlan, Mariga Alfred Mugambi, Yang Wenjian
Key Laboratory of Grains and Oils Quality Control and Processing, Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, 210023, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, 100048, China.
Food Chem Toxicol. 2021 May;151:112111. doi: 10.1016/j.fct.2021.112111. Epub 2021 Mar 11.
Biologically active peptides released by proteins are important in regulating immunity. The purpose of this study was to isolate and purify an immunologically active peptide from Hericium erinaceus (H. erinaceus) and to explore its effect on cytokine secretion and differentiation of macrophages. An active peptide with an amino acid sequence, Lys-Ser-Pro-Leu-Tyr (KSPLY) was obtained from H. erinaceus protein by ultrafiltration combined with multistage chromatography separation and identification technology. Subsequently, it was confirmed that the synthetic peptide KSPLY had a good immunomodulatory activity at a concentration of 100 μmol/L and could promote the secretion of NO, IL-1β, IL-6 and TNF-α by macrophages. The effects of KSPLY on M1 macrophages and M2 macrophages were also studied. Results showed that KSPLY inhibited the secretion of NO and IL-6 by M1 macrophages and promoted the tendency of M2 macrophages to transform to M1 macrophages. Therefore, it can be concluded that KSPLY is an effective immunomodulatory peptide that may be beneficial in cancer treatment and human health improvement.
蛋白质释放的生物活性肽在调节免疫方面很重要。本研究的目的是从猴头菇中分离和纯化一种具有免疫活性的肽，并探讨其对巨噬细胞细胞因子分泌和分化的影响。通过超滤结合多级色谱分离鉴定技术，从猴头菇蛋白中获得了一种氨基酸序列为Lys-Ser-Pro-Leu-Tyr（KSPLY）的活性肽。随后证实，合成肽KSPLY在浓度为100μmol/L时具有良好的免疫调节活性，可促进巨噬细胞分泌NO、IL-1β、IL-6和TNF-α。还研究了KSPLY对M1巨噬细胞和M2巨噬细胞的影响。结果表明，KSPLY抑制M1巨噬细胞分泌NO和IL-6，并促进M2巨噬细胞向M1巨噬细胞转化的趋势。因此，可以得出结论，KSPLY是一种有效的免疫调节肽，可能对癌症治疗和改善人类健康有益。
