Li Shuchan, An Miaoqing, Zhao Yuxuan, Zhao Wenjun, Li Pan, Du Bing
College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Food Chem X. 2024 Sep 27;24:101863. doi: 10.1016/j.fochx.2024.101863. eCollection 2024 Dec 30.
Sturgeons (), ancient fish known for their caviar, produce underutilized by-products like protein-rich cartilage, which is a source of high-quality bioactive peptides. This study investigates immunomodulatory peptides from sturgeon cartilage hydrolysates mechanisms. The study found that sturgeon cartilage hydrolysate F2-7 and its key peptides(DHVPLPLP and HVPLPLP)significantly promoted RAW267.4 cell proliferation, NO release, and phagocytosis ( < 0.001).Additionally, western blotting confirmed that F2-7 enhances immune response by increasing the expression of P-IKKα/β, IΚΚ, p65, and P-p65 proteins in the NF-κB signalling pathway ( < 0.01). Molecular docking further demonstrated that DHVPLPLP and HVPLPLP bind to NF-κB pathway proteins via hydrogen bonding, with low estimated binding energies (-2.75 and -1.64; -6.04 and -4.75 kcal/mol), thus establishing their role as key immune peptides in F2-7. Therefore, DHVPLPLP and HVPLPLP have the potential to be developed as dietary supplements for immune enhancement. Their ability to enhance immune function provides a theoretical basis for novel immune supplements.
鲟鱼（），一种以鱼子酱闻名的古老鱼类，会产生一些未得到充分利用的副产品，比如富含蛋白质的软骨，而软骨是优质生物活性肽的来源。本研究调查了鲟鱼软骨水解产物的免疫调节肽机制。研究发现，鲟鱼软骨水解产物F2-7及其关键肽（DHVPLPLP和HVPLPLP）显著促进了RAW267.4细胞的增殖、一氧化氮释放和吞噬作用（<0.001）。此外，蛋白质印迹法证实，F2-7通过增加NF-κB信号通路中P-IKKα/β、IΚΚ、p65和P-p65蛋白的表达来增强免疫反应（<0.01）。分子对接进一步证明，DHVPLPLP和HVPLPLP通过氢键与NF-κB通路蛋白结合，估计结合能较低（-2.75和-1.64；-6.04和-4.75千卡/摩尔），从而确立了它们作为F2-7中关键免疫肽的作用。因此，DHVPLPLP和HVPLPLP有潜力被开发为增强免疫力的膳食补充剂。它们增强免疫功能的能力为新型免疫补充剂提供了理论依据。
