猴头菇多糖及其对 RAW 264.7 巨噬细胞的免疫调节作用。
Polysaccharides from Hericium erinaceus and its immunomodulatory effects on RAW 264.7 macrophages.
机构信息
Department of Pharmacy, Hebei North University, Zhangjiakou, China.
Department of Pharmacy, Hebei North University, Zhangjiakou, China; Tianjin Beichen Traditional Chinese Medicine Hospital.
出版信息
Int J Biol Macromol. 2024 Oct;278(Pt 3):134947. doi: 10.1016/j.ijbiomac.2024.134947. Epub 2024 Aug 20.
This study aimed to optimize the extraction of Hericium erinaceus polysaccharides (HEP) using ultrasound-assisted enzymatic extraction combined with Plackett-Burman design (PBD) and response surface methodology (RSM). The optimal extraction conditions were identified as: 33 min extraction time, 30:1 liquid to material ratio, 38 °C extraction temperature, 9 g/kg cellulase amount, pH 4, and 20 % ethanol concentration. Under these conditions, the extraction yield of HEP was 5.87 ± 0.16 %, consistent with the predicted results. Additionally, the potential immunomodulatory activity of HEP on RAW 264.7 macrophage was evaluated. The results revealed that HEP improved the immunostimulatory activity of RAW264.7 cells, evident from increased production of IL-6 and TNF-α. These findings suggest that HEP is capable of enhancing the immune activity of RAW 264.7 macrophage.
本研究旨在通过超声辅助酶解结合 Plackett-Burman 设计（PBD）和响应面法（RSM）优化猴头菇多糖（HEP）的提取。确定的最佳提取条件为：提取时间 33min，液料比 30:1，提取温度 38°C，纤维素酶用量 9g/kg，pH4，乙醇浓度 20%。在此条件下，HEP 的提取率为 5.87±0.16%，与预测结果一致。此外，还评估了 HEP 对 RAW 264.7 巨噬细胞的潜在免疫调节活性。结果表明，HEP 提高了 RAW264.7 细胞的免疫刺激活性，这可从 IL-6 和 TNF-α 的产生增加得到证明。这些发现表明 HEP 能够增强 RAW 264.7 巨噬细胞的免疫活性。
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