小檗碱对 2 型糖尿病 Goto-Kakizaki 大鼠高血糖及肠道微生物组成的影响。
Effect of berberine on hyperglycaemia and gut microbiota composition in type 2 diabetic Goto-Kakizaki rats.
机构信息
Department of Endocrinology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, Anhui Province, China.
Department of Infectious Diseases, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, Anhui Province, China.
出版信息
World J Gastroenterol. 2021 Feb 28;27(8):708-724. doi: 10.3748/wjg.v27.i8.708.
BACKGROUND
A recent investigation showed that the prevalence of type 2 diabetes mellitus (T2DM) is 12.8% among individuals of Han ethnicity. Gut microbiota has been reported to play a central role in T2DM. Goto-Kakizaki (GK) rats show differences in gut microbiota compared to non-diabetic rats. Previous studies have indicated that berberine could be successfully used to manage T2DM. We sought to understand its hypoglycaemic effect and role in the regulation of the gut microbiota.
AIM
To determine whether berberine can regulate glucose metabolism in GK rats the gut microbiota.
METHODS
GK rats were acclimatized for 1 wk. The GK rats were randomly divided into three groups and administered saline (Mo), metformin (Me), or berberine (Be). The observation time was 8 wk, and weight, fasting blood glucose (FBG), insulin, and glucagon-like peptide-1 (GLP-1) were measured. Pancreatic tissue was observed for pathological changes. Additionally, we sequenced the 16S rRNA V3-V4 region of the gut microbiota and analysed the structure.
RESULTS
Compared with the Mo group, the Me and Be groups displayed significant differences in FBG ( < 0.01) and GLP-1 ( < 0.05). A significant decrease in weight and homeostatic model assessment-insulin resistance was noted in the Be group compared with those in the Me group ( < 0.01). The pancreatic islets of the Me- and Be-treated rats showed improvement in number, shape, and necrosis compared with those of Mo-treated rats. A total of 580 operational taxonomic units were obtained in the three groups. Compared to the Mo group, the Me and Be groups showed a shift in the structure of the gut microbiota. Correlation analysis indicated that FBG was strongly positively correlated with Clostridia_UCG-014 ( < 0.01) and negatively correlated with ( < 0.01). Body weight showed a positive correlation with ( < 0.01) and a negative correlation with ( < 0.01). Importantly, our results demonstrated that Me and Be could significantly decrease ( < 0.01) and the / ratio ( < 0.01). Furthermore, ( < 0.01; < 0.05) was significantly decreased in the Me and Be groups, and ( < 0.01) was significantly increased.
CONCLUSION
Berberine has a substantial effect in improving metabolic parameters and modulating the gut microbiota composition in T2DM rats.
背景
最近的一项调查显示，汉族人群中 2 型糖尿病（T2DM）的患病率为 12.8%。肠道微生物群在 T2DM 中起着核心作用。与非糖尿病大鼠相比，Goto-Kakizaki（GK）大鼠的肠道微生物群存在差异。先前的研究表明，小檗碱可成功用于治疗 T2DM。我们试图了解其降血糖作用及其在调节肠道微生物群中的作用。
目的
确定小檗碱是否可以调节 GK 大鼠的葡萄糖代谢和肠道微生物群。
方法
将 GK 大鼠适应环境 1 周。将 GK 大鼠随机分为三组，分别给予生理盐水（Mo）、二甲双胍（Me）或小檗碱（Be）。观察时间为 8 周，测量体重、空腹血糖（FBG）、胰岛素和胰高血糖素样肽-1（GLP-1）。观察胰腺组织的病理变化。此外，我们对肠道微生物群的 16S rRNA V3-V4 区进行测序，并分析结构。
结果
与 Mo 组相比，Me 和 Be 组 FBG（<0.01）和 GLP-1（<0.05）差异有统计学意义。与 Me 组相比，Be 组体重和稳态模型评估-胰岛素抵抗显著降低（<0.01）。与 Mo 组相比，Me 和 Be 处理组的大鼠胰岛数量、形态和坏死均有所改善。三组共获得 580 个操作分类单元。与 Mo 组相比，Me 和 Be 组的肠道微生物群结构发生了变化。相关性分析表明，FBG 与 Clostridia_UCG-014 呈强正相关（<0.01），与呈负相关（<0.01）。体重与呈正相关（<0.01），与呈负相关（<0.01）。重要的是，我们的结果表明，Me 和 Be 可以显著降低（<0.01）和/比值（<0.01）。此外，Me 和 Be 组的（<0.01；<0.05）显著降低，（<0.01）显著升高。
结论
小檗碱在改善 T2DM 大鼠代谢参数和调节肠道微生物群组成方面具有显著作用。
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