Batt R M, McLean L, Riley J E
Department of Veterinary Pathology, University of Liverpool, UK.
Gut. 1988 Apr;29(4):473-82. doi: 10.1136/gut.29.4.473.
Dogs with naturally occurring aerobic or anaerobic bacterial overgrowth have been examined before and after antibiotic therapy in order to assess reversibility of damage to the jejunal mucosa. Histological changes in peroral jejunal biopsies were relatively minor before and after treatment, but sucrose density gradient centrifugation revealed specific biochemical abnormalities that responded to antibiotic therapy. Aerobic overgrowth was initially associated with a marked loss of the main brush border component of alkaline phosphatase activity; this recovered following treatment, suggesting that aerobic bacteria may cause reversible damage to the hydrophobic region of the brush border membrane. In contrast, anaerobic overgrowth was initially associated with a marked reduction in brush border density, indicative of a considerable fall in the glycoprotein-to-lipid ratio of the membrane. Density increased from 1.17 to 1.21 g/ml after antibiotic therapy, consistent with recovery from this relatively severe damage to the brush border caused by anaerobic bacteria. Reductions in soluble and peroxisomal catalase activities which could compromise mucosal protection against free radicals in dogs with aerobic overgrowth, and a loss of particulate malate dehydrogenase activity indicative of mitochondrial disruption in dogs with anaerobic overgrowth, were also reversed after treatment. These findings indicate that aerobic and anaerobic bacterial overgrowth can result in contrasting but potentially reversible damage to the jejunal mucosa which would not be detected by conventional investigative procedures.
对患有自然发生的需氧或厌氧细菌过度生长的犬只在抗生素治疗前后进行了检查，以评估空肠黏膜损伤的可逆性。治疗前后经口空肠活检的组织学变化相对较小，但蔗糖密度梯度离心显示了对抗生素治疗有反应的特定生化异常。需氧菌过度生长最初与碱性磷酸酶活性的主要刷状缘成分显著丧失有关；治疗后这种情况得以恢复，这表明需氧菌可能对刷状缘膜的疏水区域造成可逆性损伤。相比之下，厌氧菌过度生长最初与刷状缘密度显著降低有关，这表明膜的糖蛋白与脂质比率大幅下降。抗生素治疗后密度从1.17克/毫升增加到1.21克/毫升，这与厌氧菌对刷状缘造成的这种相对严重损伤的恢复情况一致。需氧菌过度生长的犬只中可溶性和过氧化物酶体过氧化氢酶活性降低，这可能损害黏膜对自由基的保护，而厌氧菌过度生长的犬只中颗粒状苹果酸脱氢酶活性丧失表明线粒体受到破坏，治疗后这些情况也得到了逆转。这些发现表明，需氧和厌氧菌过度生长可导致空肠黏膜形成截然不同但可能可逆的损伤，而这些损伤通过传统检查程序无法检测到。
