冷水游泳应激后皮质酮的调节及HPA轴中信号分子的变化

Modulation of corticosterone and changes of signal molecules in the HPA axis after cold water swimming stress.

作者信息

Feng Jing Hui, Sim Su Min, Park Jung Seok, Hong Jae Seung, Suh HongWon

机构信息

Department of Pharmacology and Institute of Natural Medicine, College of Medicine, Hallym University, Chun-Cheon, South Korea.

Department of Physical Education, Hallym University, Chun-Cheon, South Korea.

出版信息

Anim Cells Syst (Seoul). 2021 Feb 23;25(1):37-45. doi: 10.1080/19768354.2021.1890211.

In the present study, we examined the effect of cold-water swimming stress (CWSS) on plasma corticosterone levels. Mice were exposed to stress in 4°C for 3 mins. Plasma corticosterone (CORT) level was measured at 0, 15, and 30 min after stress stimulation. The plasma CORT level was gradually increased up to 30 min. Then we further examined the changes of several signaling molecules expression levels, such as p-ERK, p-JNK, p-P38, p-AMPKα1, p-AMPKα2, and p-mTOR, in the HPA axis. We observed that those signaling molecules were altered after stress in the HPA axis. p-ERK, p-JNK, p-P38, and p-mTOR proteins expression were reduced by CWSS in the HPA axis. However, the phosphorylation of AMPKα1 and AMPKα2 were activated after CWSS in the HPA axis. Our results suggest that the upregulation of plasma CORT level induced by CWSS may be modulated by the those signaling molecules.

在本研究中，我们检测了冷水游泳应激（CWSS）对血浆皮质酮水平的影响。将小鼠置于4°C环境中应激3分钟。在应激刺激后0、15和30分钟测量血浆皮质酮（CORT）水平。血浆CORT水平在30分钟内逐渐升高。然后我们进一步检测了下丘脑-垂体-肾上腺（HPA）轴中几种信号分子表达水平的变化，如磷酸化细胞外信号调节激酶（p-ERK）、磷酸化应激活化蛋白激酶（p-JNK）、磷酸化p38丝裂原活化蛋白激酶（p-P38）、磷酸化5'-腺苷酸活化蛋白激酶α1（p-AMPKα1）、磷酸化5'-腺苷酸活化蛋白激酶α2（p-AMPKα2）和磷酸化哺乳动物雷帕霉素靶蛋白（p-mTOR）。我们观察到这些信号分子在HPA轴应激后发生了改变。在HPA轴中，CWSS使p-ERK、p-JNK、p-P38和p-mTOR蛋白表达降低。然而，在HPA轴中，CWSS后AMPKα1和AMPKα2的磷酸化被激活。我们的结果表明，CWSS诱导的血浆CORT水平上调可能受这些信号分子的调节。