Jiang Tianyue, Liu Chendan, Xu Xiao, He Bingfang, Mo Ran
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Center of Advanced Pharmaceuticals and Biomaterials, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
Front Bioeng Biotechnol. 2021 Feb 26;9:598050. doi: 10.3389/fbioe.2021.598050. eCollection 2021.
Exploiting enzyme-catalyzed reactions to manipulate molecular assembly has been considered as an attractive bottom-up nanofabrication approach to developing a variety of nano-, micro-, and macroscale structures. Upon enzymatic catalysis, peptides and their derivatives transform to assemblable building blocks that form ordered architecture by non-covalent interactions. The peptide assemblies with unique characteristics have great potential for applications in bionanotechnology and biomedicine. In this mini review, we describe typical mechanisms of the protease-instructed peptide assembly bond-cleaving or bond-forming reactions, and outline biomedical applications of the peptide assemblies, such as drug depot, sustained release, controlled release, gelation-regulated cytotoxicity, and matrix construction.
利用酶催化反应来操纵分子组装被认为是一种有吸引力的自下而上的纳米制造方法，可用于开发各种纳米、微米和宏观尺度的结构。在酶催化作用下，肽及其衍生物转化为可组装的构建块，这些构建块通过非共价相互作用形成有序结构。具有独特特性的肽组装体在生物纳米技术和生物医学领域具有巨大的应用潜力。在本综述中，我们描述了蛋白酶指导的肽组装的典型机制——断键或成键反应，并概述了肽组装体的生物医学应用，如药物储存、缓释、控释、凝胶化调节细胞毒性和基质构建。
