Liu Hao, Shukla Pratyoosh
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
ACS Omega. 2021 Feb 26;6(9):6055-6063. doi: 10.1021/acsomega.0c05744. eCollection 2021 Mar 9.
Microbial endoglucanases belonging to the β-1-4 glycosyl hydrolase family are useful enzymes due to their vast industrial applications in pulp and paper industries and biorefinery. They convert lignocellulosic substrates to soluble sugars and help in the biodegradation process. Various biocomputational tools can be utilized to understand the catalytic activity, reaction kinetics, complexity of active sites, and chemical behavior of enzyme complexes in reactions. This might be helpful in increasing productivity and cost reduction in industries. The present review gives an overview of some interesting aspects of enzyme design, including computational techniques such as molecular dynamics simulation, homology modeling, mutational analysis, etc., toward enhancing the quality of these enzymes. Moreover, the review also covers the aspects of synthetic biology, which could be helpful in faster and reliable development of useful enzymes with desired characteristics and applications. Finally, the review also deciphers the utilization of endoglucanases in biodegradation and emphasizes the use of diversified protein engineering tools and the modification of metabolic pathways for enzyme engineering.
属于β-1-4糖基水解酶家族的微生物内切葡聚糖酶是有用的酶，因为它们在制浆造纸工业和生物精炼中有广泛的工业应用。它们将木质纤维素底物转化为可溶性糖，并有助于生物降解过程。可以利用各种生物计算工具来了解酶复合物在反应中的催化活性、反应动力学、活性位点的复杂性和化学行为。这可能有助于提高工业生产率和降低成本。本综述概述了酶设计的一些有趣方面，包括分子动力学模拟、同源建模、突变分析等计算技术，以提高这些酶的质量。此外，该综述还涵盖了合成生物学的各个方面，这可能有助于更快、可靠地开发具有所需特性和应用的有用酶。最后，该综述还解读了内切葡聚糖酶在生物降解中的应用，并强调了使用多样化的蛋白质工程工具和修饰代谢途径进行酶工程。
