Lee Dongseob, Lee Jungwon, Ahn Sun-Hee, Song Woosub, Li Ling, Seol Yang-Jo, Lee Yong-Moo, Koo Ki-Tae
Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University and Seoul National University Dental Hospital, Seoul, Korea.
Department of Periodontology, Seoul National University Dental Hospital, Seoul, Korea.
J Periodontal Implant Sci. 2024 Feb;54(1):13-24. doi: 10.5051/jpis.2203580179. Epub 2023 May 9.
This study investigated the adjunctive effect of light-emitting diodes (LEDs) in the treatment of experimental periodontitis.
Experimental periodontitis was induced by placing ligatures around the mandibular second, third, and fourth premolars of 6 beagles for 3 months. After ligature removal, periodontitis progressed spontaneously for 2 months. The animals' hemimandibles were allocated among the following 3 groups: 1) no treatment (control), 2) scaling and root planing (SRP), and 3) SRP with LED irradiation at 470-nm and 630-nm wavelengths (SRP/LED). The probing pocket depth (PPD) and gingival recession (GR) were measured at baseline, 6 weeks, and 12 weeks. The clinical attachment level (CAL) was calculated. After 12 weeks, histological and histomorphometric assessments were performed. The distances from the gingival margin to the apical extent of the junctional epithelium (E) and to the connective tissue (CT) attachment were measured, as was the total length of soft tissue (ST).
PPD and CAL increased at 12 weeks compared with baseline in the control group (6.31±0.43 mm to 6.93±0.50 mm, and 6.46±0.60 mm to 7.61±0.78 mm, respectively). PPD and CAL decreased at 12 weeks compared with baseline in the SRP group (6.01±0.59 to 4.81±0.65 mm, and 6.51±0.98 to 5.39±0.93 mm, respectively). PPD and CAL decreased at 12 weeks compared with baseline in the SRP/LED group (6.03±0.39 to 4.46±0.47 mm, and 6.11±0.47 to 4.78±0.57 mm, respectively). The E/ST and CT/ST ratios significantly differed among the 3 groups (<0.05). The clinical parameters and histologic findings demonstrated that 470-nm and 630-nm wavelength LED irradiation accompanying SRP could improve treatment results.
Within the study limitations, 470 nm and 630 nm wavelength LED irradiation might provide additional benefits for periodontitis treatment.
本研究调查了发光二极管（LED）在实验性牙周炎治疗中的辅助作用。
通过在6只比格犬的下颌第二、第三和第四前磨牙周围放置结扎丝3个月来诱导实验性牙周炎。去除结扎丝后，牙周炎自发进展2个月。将动物的半侧下颌骨分配到以下3组中：1）不治疗（对照组），2）龈下刮治和根面平整（SRP），3）SRP联合470纳米和630纳米波长的LED照射（SRP/LED）。在基线、6周和12周时测量探诊深度（PPD）和牙龈退缩（GR）。计算临床附着水平（CAL）。12周后，进行组织学和组织形态计量学评估。测量从牙龈边缘到结合上皮顶端范围（E）以及到结缔组织（CT）附着的距离，以及软组织（ST）的总长度。
与基线相比，对照组12周时PPD和CAL增加（分别从6.31±0.43毫米增加到6.93±0.50毫米，以及从6.46±0.60毫米增加到7.61±0.78毫米）。与基线相比，SRP组12周时PPD和CAL降低（分别从6.01±0.59降低到4.81±0.65毫米，以及从6.51±0.98降低到5.39±0.93毫米）。与基线相比，SRP/LED组12周时PPD和CAL降低（分别从6.03±0.39降低到4.46±0.47毫米，以及从6.11±0.47降低到4.78±0.57毫米）。3组之间E/ST和CT/ST比值有显著差异（<0.05）。临床参数和组织学结果表明，SRP联合470纳米和630纳米波长的LED照射可改善治疗效果。
在本研究的局限性范围内，470纳米和630纳米波长的LED照射可能为牙周炎治疗带来额外益处。
