Division of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, India.
Nat Prod Res. 2022 Mar;36(6):1663-1667. doi: 10.1080/14786419.2021.1899174. Epub 2021 Mar 15.
The potential strategy to prevent bacterial pathogenicity is disabling quorum sensing circuits with structural mimicking molecules. Here, we analyzed a synthetic molecule isoeugenol, for inhibition of quorum sensing regulated phenotype and biofilm formation. Isoeugenol was an effective inhibitor, i.e., more than 70% of virulence factors were inhibited including pyocyanin, rhamnolipid, exopolysaccharide, swarming motility and biofilm formation. Interestingly, these quorum sensing regulated phenotypes in PAO1 were inhibited without affecting the planktonic cells. Moreover, the presence of isoeugenol exhibited more than 70% inhibition of biofilm formation through inhibition of the quorum sensing systems. Furthermore, docking studies suggest that isoeugenol bound to the quorum sensor regulators such as LasI, LasR PqsE and SidA with considerable binding interactions. Our results demonstrate the utility of isoeugenol as a blocker of quorum sensing, which will be functioning as an antivirulence compound.
防止细菌致病性的潜在策略是用结构模拟分子使群体感应电路失活。在这里，我们分析了一种合成分子异丁香酚，以抑制群体感应调节的表型和生物膜形成。异丁香酚是一种有效的抑制剂，即超过 70%的毒力因子被抑制，包括绿脓菌素、鼠李糖脂、胞外多糖、群集运动和生物膜形成。有趣的是，这些在 PAO1 中受群体感应调节的表型在不影响浮游细胞的情况下被抑制。此外，异丁香酚的存在通过抑制群体感应系统表现出超过 70%的生物膜形成抑制作用。此外，对接研究表明，异丁香酚与群体感应调节剂如 LasI、LasR PqsE 和 SidA 结合，具有相当大的结合相互作用。我们的结果表明，异丁香酚可用作群体感应的阻断剂，这将作为一种抗毒力化合物发挥作用。
