Wang Ying-Jie, Wang Fang, Jiang Ming-Hua, Xu Kai-Zhong, Dar Owias Iqbal, Tang Shi, Liu Lan, Chen Sen-Hua, Jia Ai-Qun
Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou, 570228, China.
School of Marine Sciences, Sun Yat-Sen University, Southern Marine Sciences and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, China.
Curr Microbiol. 2025 Mar 27;82(5):215. doi: 10.1007/s00284-025-04202-4.
The emergence of multidrug-resistant Chromobacterium violaceum, an opportunistic pathogen, poses a significant threat to human, animal, and environmental health, underscoring the urgent need for innovative strategies. Marine-derived natural compounds have gained attention as a promising source of quorum sensing inhibitors (QSIs) that can attenuate C. violaceum virulence without inducing resistance. This study reports, for the first time, the anti-quorum sensing (anti-QS) and anti-biofilm activities of oxirapentyn A, one marine natural compound, against C. violaceum. Results demonstrate oxirapentyn A (200 μg/mL) significantly inhibits biofilm formation, violacein production, and hemolysin synthesis by 48.8, 21.7, and 22.3%, respectively. Scanning electron microscopy (SEM) further corroborated the disruption of biofilm architecture. LC-MS analysis revealed a concentration-dependent reduction in the production of N-decanoyl-homoserine lactone (C-HSL), a key QS signaling molecule. Furthermore, RT-qPCR analysis indicated oxirapentyn A downregulated critical QS-related genes (cviI, cviR, vioA, chiA, and pykF) by 20.7, 36.6, 31.1, 66.6, and 30.7%, respectively. Notably, in vivo experiments demonstrated that oxirapentyn A significantly improved the survival of Galleria mellonella larvae infected with C. violaceum. Collectively, these findings highlight oxirapentyn A as a novel QSI with dual anti-QS and biofilm-disrupting activities, offering a promising strategy to combat drug-resistant bacterial infections.
多重耐药性紫色色杆菌作为一种机会致病菌的出现，对人类、动物和环境健康构成了重大威胁，凸显了对创新策略的迫切需求。海洋来源的天然化合物作为群体感应抑制剂（QSIs）的一个有前景的来源而受到关注，这些抑制剂可以减弱紫色色杆菌的毒力而不诱导耐药性。本研究首次报道了一种海洋天然化合物奥昔拉喷汀A对紫色色杆菌的抗群体感应（抗QS）和抗生物膜活性。结果表明，奥昔拉喷汀A（200μg/mL）分别显著抑制生物膜形成、紫色菌素产生和溶血素合成48.8%、21.7%和22.3%。扫描电子显微镜（SEM）进一步证实了生物膜结构的破坏。液相色谱-质谱分析显示关键的群体感应信号分子N-癸酰基高丝氨酸内酯（C-HSL）的产生呈浓度依赖性降低。此外，逆转录定量聚合酶链反应（RT-qPCR）分析表明，奥昔拉喷汀A分别下调关键的群体感应相关基因（cviI、cviR、vioA、chiA和pykF）20.7%、36.6%、31.1%、66.6%和30.7%。值得注意的是，体内实验表明，奥昔拉喷汀A显著提高了感染紫色色杆菌的大蜡螟幼虫的存活率。总的来说，这些发现突出了奥昔拉喷汀A作为一种具有双重抗QS和生物膜破坏活性的新型群体感应抑制剂，为对抗耐药细菌感染提供了一种有前景的策略。
