Kieffer M, Frazier P J, Daniels N W, Coombs R R
Clin Exp Immunol. 1982 Dec;50(3):651-60.
The mixed reverse solid phase passive antiglobulin haemadsorption test (MRSPAH) and the enzyme linked immunosorbant assay (ELISA) were found equally sensitive and fitted for the measurement of serum IgG antibodies against alcohol soluble gliadins. Using the ELISA method, three coeliac sera with elevated antibody titres against gliadins and two control sera with low titres were tested for IgG antibodies against the main groups of wheat proteins (acetic acid soluble glutenins, salt soluble albumins and globulins and alcohol soluble gliadins), eight fractions of gliadin and the alcohol soluble proteins of barley, rye, oat, maize and rice. As rice contained little alcohol soluble protein, a test against acid soluble rice proteins was included. In all three patient sera, titres higher than or equal to that for crude gliadin were found for wheat glutenin and for gliadin fractions seven and eight, both containing alpha gliadins. Similar high titres were found when these coeliac sera were tested against rye, barley and oat prolamines. Maize prolamines gave only low titres and no antibodies could be detected against rice proteins, in line with the tolerance of these latter two cereals by patients with coeliac disease. It would appear that sera from coeliac patients react with more than one antigenic fraction of protein in wheat and other cereals. Also sera from two normal persons appeared to have the same spectrum of reactivity against these cereal proteins as did the three sera from coeliac patients. The titres in normal sera were however much lower.
混合反向固相被动抗球蛋白血细胞吸附试验（MRSPAH）和酶联免疫吸附测定（ELISA）被发现同样敏感，适用于测量血清中针对醇溶性麦醇溶蛋白的IgG抗体。使用ELISA方法，对三份针对麦醇溶蛋白抗体滴度升高的乳糜泻血清和两份低滴度对照血清进行检测，以测定其针对小麦主要蛋白质组（乙酸溶性谷蛋白、盐溶性白蛋白和球蛋白以及醇溶性麦醇溶蛋白）、八种麦醇溶蛋白组分以及大麦、黑麦、燕麦、玉米和水稻的醇溶性蛋白质的IgG抗体。由于水稻含有的醇溶性蛋白质很少，因此还进行了针对酸溶性水稻蛋白质的检测。在所有三份患者血清中，发现针对小麦谷蛋白以及含有α-麦醇溶蛋白的麦醇溶蛋白组分7和8的滴度高于或等于粗麦醇溶蛋白的滴度。当用这些乳糜泻血清检测黑麦、大麦和燕麦醇溶蛋白时，也发现了类似的高滴度。玉米醇溶蛋白的滴度很低，并且未检测到针对水稻蛋白质的抗体，这与乳糜泻患者对后两种谷物的耐受性一致。乳糜泻患者的血清似乎与小麦和其他谷物中蛋白质的多个抗原组分发生反应。另外，两名正常人的血清对这些谷物蛋白质的反应谱似乎与三份乳糜泻患者血清相同。然而，正常血清中的滴度要低得多。
