Zhao Fengqin, Wang Chuang, Sun Dongyu, Zhang Linlin, Wang Zhiyue, Piao Jun, Piao Jingai, Jin Mei
Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian, 116029, China.
Environ Sci Pollut Res Int. 2021 Jul;28(28):37918-37928. doi: 10.1007/s11356-021-12457-0. Epub 2021 Mar 15.
The morphology and oxidation state of arsenic in its compounds affects the skin cell toxicity. Accordingly, the present study was conducted to explore the effects of two different arsenic compounds on the proliferation and survival of Liaoning cashmere goat skin fibroblasts. Based on MTT assay results, at 24 h, the proliferation concentration, critical concentration, and half inhibitory concentration (IC50) of sodium arsenite were 0.50, 5.00, and 45.66 μmol/L, respectively. The corresponding values for dimethyl arsenic acid were 0.85, 1.00, and 38.68 mmol/L. Immunofluorescence, transmission electron microscopy, and mitochondria membrane potential (MMP) assays showed that sodium arsenite promotes microtubule polymerization and increases MMP, while cells treated with dimethyl arsenic acid exhibited cytoskeletal collapse and decreased MMP. In the IC50 groups for both arsenic agents, the cytoskeletons collapsed, microtubules were gathered into bundles, and MMP was significantly decreased. Dimethyl arsenic acid had a stronger effect on MMP than sodium arsenite. Flow cytometry revealed a slightly lower occurrence of apoptosis in the sodium arsenite proliferation group, while it was slightly increased in the dimethyl arsenic acid proliferation group. Apoptosis was increased more significantly in the sodium arsenite IC50 group than in the dimethyl arsenic acid IC50 group. These results indicate that the differences in cell proliferation and cytotoxicity induced by inorganic and organic arsenic are related to their effects on cellular structures.
砷化合物中砷的形态和氧化态会影响皮肤细胞毒性。因此，本研究旨在探讨两种不同砷化合物对辽宁绒山羊皮肤成纤维细胞增殖和存活的影响。基于MTT检测结果，在24小时时，亚砷酸钠的增殖浓度、临界浓度和半抑制浓度（IC50）分别为0.50、5.00和45.66μmol/L。二甲基砷酸的相应值分别为0.85、1.00和38.68mmol/L。免疫荧光、透射电子显微镜和线粒体膜电位（MMP）检测表明，亚砷酸钠促进微管聚合并增加MMP，而用二甲基砷酸处理的细胞表现出细胞骨架塌陷和MMP降低。在两种砷剂的IC50组中，细胞骨架塌陷，微管聚集成束，MMP显著降低。二甲基砷酸对MMP的影响比亚砷酸钠更强。流式细胞术显示，亚砷酸钠增殖组的凋亡发生率略低，而二甲基砷酸增殖组的凋亡发生率略有增加。亚砷酸钠IC50组的凋亡增加比二甲基砷酸IC50组更显著。这些结果表明，无机砷和有机砷诱导的细胞增殖和细胞毒性差异与其对细胞结构的影响有关。
