• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

敲除本氏烟中的 SOBIR1 会使转 Cf-4 受体样蛋白失去功能。

Knocking out SOBIR1 in Nicotiana benthamiana abolishes functionality of transgenic receptor-like protein Cf-4.

机构信息

Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

出版信息

Plant Physiol. 2021 Mar 15;185(2):290-294. doi: 10.1093/plphys/kiaa047.

DOI:10.1093/plphys/kiaa047
PMID:33721902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8133582/
Abstract

Knocking out SOBIR1 in by CRISPR/Cas9, abolishes the functionality of the transgenic receptor-like protein Cf-4, recognizing the Avr4 effector of the fungus .

摘要

通过 CRISPR/Cas9 敲除 中的 SOBIR1 ，废除了转基因受体样蛋白 Cf-4 的功能，该蛋白识别真菌 的 Avr4 效应子。

相似文献

1
Knocking out SOBIR1 in Nicotiana benthamiana abolishes functionality of transgenic receptor-like protein Cf-4.敲除本氏烟中的 SOBIR1 会使转 Cf-4 受体样蛋白失去功能。
Plant Physiol. 2021 Mar 15;185(2):290-294. doi: 10.1093/plphys/kiaa047.
2
Using forward genetics in Nicotiana benthamiana to uncover the immune signaling pathway mediating recognition of the Xanthomonas perforans effector XopJ4.利用拟南芥中的正向遗传学揭示介导黄单胞菌效应因子 XopJ4 识别的免疫信号通路。
New Phytol. 2019 Jan;221(2):1001-1009. doi: 10.1111/nph.15411. Epub 2018 Aug 29.
3
L-type lectin receptor kinases in Nicotiana benthamiana and tomato and their role in Phytophthora resistance.本氏烟草和番茄中的L型凝集素受体激酶及其在疫霉抗性中的作用。
J Exp Bot. 2015 Nov;66(21):6731-43. doi: 10.1093/jxb/erv379. Epub 2015 Aug 5.
4
The F-box protein ACRE189/ACIF1 regulates cell death and defense responses activated during pathogen recognition in tobacco and tomato.F-box蛋白ACRE189/ACIF1调控烟草和番茄在病原体识别过程中激活的细胞死亡和防御反应。
Plant Cell. 2008 Mar;20(3):697-719. doi: 10.1105/tpc.107.056978. Epub 2008 Mar 28.
5
Regions of the Cf-9B disease resistance protein able to cause spontaneous necrosis in Nicotiana benthamiana lie within the region controlling pathogen recognition in tomato.Cf-9B抗病蛋白中能够在本氏烟草中引起自发坏死的区域，位于番茄中控制病原体识别的区域内。
Mol Plant Microbe Interact. 2009 Oct;22(10):1214-26. doi: 10.1094/MPMI-22-10-1214.
6
Optimized agroinfiltration and virus-induced gene silencing to study Ve1-mediated Verticillium resistance in tobacco.优化的农杆菌浸润和病毒诱导的基因沉默技术用于研究烟草中 Ve1 介导的黄萎病抗性。
Mol Plant Microbe Interact. 2013 Feb;26(2):182-90. doi: 10.1094/MPMI-06-12-0161-R.
7
An EFR-Cf-9 chimera confers enhanced resistance to bacterial pathogens by SOBIR1- and BAK1-dependent recognition of elf18.EFR-Cf-9 嵌合体通过 SOBIR1 和 BAK1 依赖的 elf18 识别赋予对细菌病原体的增强抗性。
Mol Plant Pathol. 2019 Jun;20(6):751-764. doi: 10.1111/mpp.12789. Epub 2019 Apr 1.
8
Glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato and Nicotiana benthamiana leaf epidermis.甘油-3-磷酸酰基转移酶 6 控制丝状病原体的相互作用和番茄及本氏烟叶片表皮的细胞壁特性。
New Phytol. 2019 Aug;223(3):1547-1559. doi: 10.1111/nph.15846. Epub 2019 May 10.
9
Naturally occurring broad-spectrum powdery mildew resistance in a Central American tomato accession is caused by loss of mlo function.中美洲番茄种质中天然存在的广谱白粉病抗性是由mlo功能丧失引起的。
Mol Plant Microbe Interact. 2008 Jan;21(1):30-9. doi: 10.1094/MPMI-21-1-0030.
10
Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato.对Avr9/Cf-9快速诱导基因的功能分析鉴定出一种蛋白激酶ACIK1，它对于番茄中完全依赖Cf-9的抗病性至关重要。
Plant Cell. 2005 Jan;17(1):295-310. doi: 10.1105/tpc.104.026013. Epub 2004 Dec 14.

引用本文的文献

1
Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins.来自不同界的叶部松树病原体共享引发防御的效应蛋白。
Mol Plant Pathol. 2025 Mar;26(3):e70065. doi: 10.1111/mpp.70065.
2
Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana.不同亚家族的类受体细胞质激酶差异调节烟草原生质体 SOBR1/BAK1 介导的免疫反应。
Nat Commun. 2024 May 21;15(1):4339. doi: 10.1038/s41467-024-48313-1.
3
Comprehensive analysis of sesame LRR-RLKs: structure, evolution and dynamic expression profiles under stress.芝麻富含亮氨酸重复序列受体样激酶的综合分析：结构、进化及胁迫下的动态表达谱
Front Plant Sci. 2024 Feb 12;15:1334189. doi: 10.3389/fpls.2024.1334189. eCollection 2024.
4
Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants.超越 Fulvia fulva（同 Cladosporium fulvum）和 Dothistroma septosporum 的基因组：这些真菌病原体与其宿主植物相互作用的新见解。
Mol Plant Pathol. 2023 May;24(5):474-494. doi: 10.1111/mpp.13309. Epub 2023 Feb 15.
5
Convergent evolution of immune receptors underpins distinct elicitin recognition in closely related Solanaceous plants.免疫受体的趋同进化为近缘茄科植物中独特的 elicitin 识别提供了基础。
Plant Cell. 2023 Mar 29;35(4):1186-1201. doi: 10.1093/plcell/koad002.
6
Receptor-like Kinases (LRR-RLKs) in Response of Plants to Biotic and Abiotic Stresses.类受体激酶（LRR-RLKs）在植物对生物和非生物胁迫的响应中
Plants (Basel). 2022 Oct 10;11(19):2660. doi: 10.3390/plants11192660.
7
Botrytis hypersensitive response inducing protein 1 triggers noncanonical PTI to induce plant cell death.葡萄孢诱导过敏反应蛋白 1 触发非典型的 PTI 诱导植物细胞死亡。
Plant Physiol. 2023 Jan 2;191(1):125-141. doi: 10.1093/plphys/kiac476.
8
The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4.辅助 NLR 免疫蛋白 NRC3 介导由细胞表面受体 Cf-4 引起的超敏细胞死亡。
PLoS Genet. 2022 Sep 22;18(9):e1010414. doi: 10.1371/journal.pgen.1010414. eCollection 2022 Sep.
9
Multiple knockout mutants reveal a high redundancy of phytotoxic compounds contributing to necrotrophic pathogenesis of Botrytis cinerea.多种敲除突变体揭示了对 Botrytis cinerea 坏死病致病性有贡献的植物毒性化合物的高度冗余性。
PLoS Pathog. 2022 Mar 3;18(3):e1010367. doi: 10.1371/journal.ppat.1010367. eCollection 2022 Mar.
10
How activated NLRs induce anti-microbial defenses in plants.植物中被激活的 NLR 如何诱导抗微生物防御。
Biochem Soc Trans. 2021 Nov 1;49(5):2177-2188. doi: 10.1042/BST20210242.

本文引用的文献

1
Generation and Molecular Characterization of CRISPR/Cas9-Induced Mutations in 63 Immunity-Associated Genes in Tomato Reveals Specificity and a Range of Gene Modifications.CRISPR/Cas9诱导番茄63个免疫相关基因突变的产生及分子特征分析揭示了其特异性和一系列基因修饰
Front Plant Sci. 2020 Feb 4;11:10. doi: 10.3389/fpls.2020.00010. eCollection 2020.
2
Plant Immunity: Thinking Outside and Inside the Box.植物免疫：超越框框思考
Trends Plant Sci. 2019 Jul;24(7):587-601. doi: 10.1016/j.tplants.2019.04.009. Epub 2019 Jun 3.
3
Kinase activity of SOBIR1 and BAK1 is required for immune signalling.SOBIR1 和 BAK1 的激酶活性是免疫信号所必需的。
Mol Plant Pathol. 2019 Mar;20(3):410-422. doi: 10.1111/mpp.12767. Epub 2019 Jan 2.
4
The Bacterial Effector AvrPto Targets the Regulatory Coreceptor SOBIR1 and Suppresses Defense Signaling Mediated by the Receptor-Like Protein Cf-4.细菌效应因子 AvrPto 靶向调节核心受体 SOBIR1 并抑制受体样蛋白 Cf-4 介导的防御信号。
Mol Plant Microbe Interact. 2018 Jan;31(1):75-85. doi: 10.1094/MPMI-08-17-0203-FI. Epub 2017 Oct 24.
5
CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants.CRISPR-P 2.0：一种用于植物基因组编辑的改进型CRISPR-Cas9工具。
Mol Plant. 2017 Mar 6;10(3):530-532. doi: 10.1016/j.molp.2017.01.003. Epub 2017 Jan 13.
6
Regulation of pattern recognition receptor signalling in plants.植物模式识别受体信号转导的调控。
Nat Rev Immunol. 2016 Sep;16(9):537-52. doi: 10.1038/nri.2016.77. Epub 2016 Aug 1.
7
An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity.RLP23-SOBIR1-BAK1 复合物介导体感 NLP 免疫。
Nat Plants. 2015 Oct 5;1:15140. doi: 10.1038/nplants.2015.140.
8
Avr4 promotes Cf-4 receptor-like protein association with the BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and plant immunity.Avr4促进Cf-4类受体蛋白与BAK1/SERK3类受体激酶结合，从而启动受体内吞作用和植物免疫反应。
New Phytol. 2016 Apr;210(2):627-42. doi: 10.1111/nph.13802. Epub 2016 Jan 14.
9
Editing plant genomes with CRISPR/Cas9.利用CRISPR/Cas9编辑植物基因组。
Curr Opin Biotechnol. 2015 Apr;32:76-84. doi: 10.1016/j.copbio.2014.11.007. Epub 2014 Nov 29.
10
Receptor-like kinase SOBIR1/EVR interacts with receptor-like proteins in plant immunity against fungal infection.受体样激酶 SOBIR1/EVR 与受体样蛋白相互作用，在植物对真菌感染的免疫反应中发挥作用。
Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):10010-5. doi: 10.1073/pnas.1220015110. Epub 2013 May 28.