环指蛋白 126：结直肠癌的潜在生物标志物。
Ring finger protein 126: a potential biomarker for colorectal cancer.
机构信息
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, PR China.
Key Laboratory of Tumor Biological Behavior of Hubei Provence, Wuhan, Hubei Province, PR China.
出版信息
Histol Histopathol. 2021 May;36(5):559-566. doi: 10.14670/HH-18-328. Epub 2021 Mar 16.
BACKGROUND
Colorectal cancer (CRC) is the most common cancer of the digestive system. However, effective therapeutic targets against CRC have not been found yet. Further, the relationship between the expression of ring finger protein 126 (RNF126) and CRC is not clear.
MATERIAL AND METHODS
The expression level of RNF126 in CRC tissues and cell lines was detected by immunohistochemical staining and western blot. Subsequently, endogenous RNF126 expression was inhibited in a CRC cell line using a short hairpin RNA. Next, the effect of RNF126 on the properties of CRC cells was studied through different experimental methods.
RESULTS
We found that the RNF126 protein was mainly localized in the cytoplasm. High RNF126 expression was observed to be an independent risk factor for poor prognosis in CRC patients. In vitro studies showed that RNF126 was able to promote the proliferation, migration, and invasion ability of CRC cells.
CONCLUSION
RNF126 acts as an oncogene during CRC development, and may serve as a novel target for CRC treatment.
背景
结直肠癌（CRC）是最常见的消化系统癌症。然而，目前尚未发现针对 CRC 的有效治疗靶点。此外，RNF126 的表达与 CRC 之间的关系尚不清楚。
材料与方法
通过免疫组织化学染色和 Western blot 检测 CRC 组织和细胞系中 RNF126 的表达水平。随后，使用短发夹 RNA 抑制 CRC 细胞系中的内源性 RNF126 表达。接下来，通过不同的实验方法研究 RNF126 对 CRC 细胞特性的影响。
结果
我们发现 RNF126 蛋白主要定位于细胞质中。高 RNF126 表达被观察为 CRC 患者预后不良的独立危险因素。体外研究表明，RNF126 能够促进 CRC 细胞的增殖、迁移和侵袭能力。
结论
RNF126 在 CRC 发展过程中起癌基因作用，可能成为 CRC 治疗的新靶点。
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