Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
Department of Veterinary Pathology, University of Ibadan, Ibadan, Oyo State, Nigeria.
J Immunoassay Immunochem. 2021 Jul 4;42(4):424-443. doi: 10.1080/15321819.2021.1895216. Epub 2021 Mar 16.
This study reports the influence of (PPR) vaccination on the clinico-pathological outcomes of PPR in the face of an outbreak. Twenty-two West African dwarf goats procured for a different study started showing early signs of PPR during acclimatization. In response, PPR vaccine was administered either intranasally with phytogenic mucoadhesive gum (Group A; = 6) or without gum (Group B; = 6); subcutaneously (Group C; = 6) or not vaccinated (Group D; = 4) and studied for 21 days. The clinical scores, hematology, serology and pathology scores were evaluated. Clinical signs of PPR were present in all groups, presenting a percentage mortality of 33%; 33%; 64% and 100% for Groups A, B, C, and D, respectively. Polycythemia and mild leukopenia were observed in all groups, and all animals were seropositive by day 7 post-vaccination. The lung consolidation scores were low in Groups A and B, compared to Group C. Histopathological lesions consistent with PPR was observed in the lymphoid organs, gastrointestinal tract, and lungs with the presence of PPR antigen as detected by immunohistochemistry. The findings suggest that intranasal vaccination with or without mucoadhesive gum may influence the outcome of PPR infection more than the subcutaneous route in the face of an outbreak.
本研究报告了在暴发期间，（PPR）疫苗接种对 PPR 的临床病理结果的影响。22 只西非小尾寒羊在适应环境期间开始出现 PPR 的早期症状，这些羊是为了进行另一项研究而购买的。作为回应，PPR 疫苗通过鼻内接种植物源黏膜黏附胶（A 组，n=6）或不使用胶（B 组，n=6）、皮下接种（C 组，n=6）或不接种（D 组，n=4）的方式进行接种，并在 21 天内进行研究。评估了临床评分、血液学、血清学和病理学评分。所有组均出现 PPR 的临床症状，A、B、C 和 D 组的死亡率分别为 33%、33%、64%和 100%。所有组均出现红细胞增多和轻度白细胞减少，所有动物在接种后第 7 天均呈血清阳性。A 组和 B 组的肺部实变评分较低，而 C 组则较高。在淋巴结、胃肠道和肺部观察到与 PPR 一致的组织病理学病变，并通过免疫组织化学检测到 PPR 抗原的存在。这些发现表明，在暴发期间，鼻内接种或不使用黏膜黏附胶可能比皮下接种更能影响 PPR 感染的结果。
