Mumin Farah Isse, Emikpe Benjamin Obukowho, Odeniyi Michael Ayodele
Faculty of Veterinary Medicine, Red Sea University, Galkaio, Somalia.
Department of Veterinary Pathology, University of Ibadan, Ibadan, Nigeria.
J Immunoassay Immunochem. 2020 May 3;41(3):311-321. doi: 10.1080/15321819.2020.1734935. Epub 2020 Mar 2.
A study was conducted to evaluate mucoadhesive property and immunomodulatory effect of phytogenic gums from and on intranasal Peste des petits ruminants (PPR) vaccination in goats and sheep in an ex-vivo and in-vivo situations. Plant gums were purified, dried and compressed into 500gm tablets. Modified shear stress measurement technique was used on freshly excised trachea and intestine tissues of goat to measure peak adhesion time. Forty eight animals (24 goats and 24 sheep) were divided into eight groups (of 3 goats and 3 sheep) and immunized intranasally with gum-vaccine combinations in two ratios (1:1, 1:2). Antibody against PPR virus was measured on day 14, 28, 42 and 56 post vaccination using H-based PPR bELISA. The peak adhesion time of the different gums was transient. PPR virus antibodies were detected in all immunized goats and sheep but not in unvaccinated control. The best percentage inhibition was recorded for Boswellia carteri-vaccine combination group at a ratio of 1:1. Administration of -PPR vaccine combination through intranasal or subcutaneous route, elicited similar antibody titre, implying that the intranasal route may be used as a non-invasive alternative delivery in PPR vaccination of small ruminants.
进行了一项研究，以评估来自[具体植物名称未给出]的植物源胶的粘膜粘附特性和免疫调节作用，以及其在体外和体内情况下对山羊和绵羊鼻内小反刍兽疫（PPR）疫苗接种的影响。将植物胶纯化、干燥并压制成500克片剂。采用改良的剪切应力测量技术，对新鲜切除的山羊气管和肠道组织进行测量，以测定峰值粘附时间。48只动物（24只山羊和24只绵羊）被分为8组（每组3只山羊和3只绵羊），并以两种比例（1:1、1:2）通过鼻内接种胶-疫苗组合。在接种疫苗后的第14、28、42和56天，使用基于H的PPR bELISA检测抗PPR病毒抗体。不同胶的峰值粘附时间是短暂的。在所有免疫的山羊和绵羊中均检测到PPR病毒抗体，但未接种疫苗的对照组中未检测到。乳香-疫苗组合组在1:1比例下的抑制百分比最高。通过鼻内或皮下途径给予[具体植物名称未给出]-PPR疫苗组合，引发了相似的抗体滴度，这意味着鼻内途径可作为小反刍兽疫PPR疫苗接种的一种非侵入性替代给药途径。
