V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
V. I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2021 Feb;170(4):528-536. doi: 10.1007/s10517-021-05101-x. Epub 2021 Mar 16.
We performed a comparative study of the proliferative potential of human mesenchymal stromal cells (MSC) from three sources (tooth pulp, adipose tissue, and Wharton's jelly) in spheroid culture; human chondroblasts served as the positive control. Histological examination revealed signs of chondrogenic differentiation in all studied cell cultures and the differences in the volume and composition of the extracellular matrix. Spheroids formed by MSC from the tooth pulp and Wharton's jelly were characterized by low content of extracellular matrix and glycosaminoglycans. Spheroids from adipose tissue MSC contained maximum amount of the extracellular matrix and high content of glycosaminoglycans. Chondrocytes produced glycosaminoglycan-enriched matrix. Type II collagen was produced by chondrocytes (to a greater extent) and adipose tissue MSC (to a lesser extent). The results of our study demonstrate that MSC from the adipose tissue under conditions of spheroid culturing exhibited maximum chondrogenic potential.
我们在球体培养中比较了来自三个来源（牙髓、脂肪组织和沃顿胶）的人骨髓间充质基质细胞（MSC）的增殖潜力；人软骨细胞作为阳性对照。组织学检查显示，所有研究的细胞培养物中都有软骨分化的迹象，细胞外基质的体积和组成也存在差异。牙髓和沃顿胶来源的 MSC 形成的球体，其细胞外基质和糖胺聚糖的含量较低。脂肪组织 MSC 形成的球体含有最多量的细胞外基质和高含量的糖胺聚糖。软骨细胞产生富含糖胺聚糖的基质。软骨细胞（更大量地）和脂肪组织 MSC（较少量地）产生 II 型胶原蛋白。我们的研究结果表明，在球体培养条件下，脂肪组织来源的 MSC 表现出最大的软骨生成潜力。
