Opt Express. 2021 Feb 15;29(4):6179-6187. doi: 10.1364/OE.419051.
In this study, surface-enhanced Raman scattering (SERS) scheme is combined with localized surface plasmon resonance (LSPR) detection on a thin gold film with stripe patterns of gold nanoparticles (GNPs) via convective self-assembly (CSA) method. The potential of dual modal plasmonic substrates was evaluated by binding 4-ABT and IgG analytes, respectively. SERS experiments presented not only a high sensitivity with a detection limit of 4.7 nM and an enhancement factor of 1.34 × 10, but an excellent reproducibility with relative standard deviation of 5.5%. It was found from plasmonic sensing experiments by immobilizing IgG onto GNP-mediated gold film that detection sensitivity was improved by more than 211%, compared with a conventional bare gold film. Our synergistic SERS-LSPR approach based on a simple and cost-effective CSA method could open a route for sensitive, reliable and reproducible dual modal detection to expand the application areas.
在这项研究中，通过对流自组装（convective self-assembly，CSA）方法，将表面增强拉曼散射（surface-enhanced Raman scattering，SERS）方案与具有金纳米粒子（gold nanoparticles，GNPs）条纹图案的薄金膜上的局域表面等离激元共振（localized surface plasmon resonance，LSPR）检测相结合。通过分别结合 4-ABT 和 IgG 分析物，评估了双模态等离子体基片的潜力。SERS 实验不仅具有高灵敏度（检测限为 4.7 nM，增强因子为 1.34×10），而且具有出色的重现性（相对标准偏差为 5.5%）。通过将 IgG 固定在介导金膜的 GNPs 上进行等离子体传感实验发现，与传统的裸金膜相比，检测灵敏度提高了 211%以上。我们基于简单且具有成本效益的 CSA 方法的协同 SERS-LSPR 方法可以为敏感、可靠和可重复的双模检测开辟一条途径，从而扩展应用领域。
