Singh A K, Ashraf M
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
J Chromatogr. 1988 Mar 18;425(2):245-55. doi: 10.1016/0378-4347(88)80029-x.
Gas chromatography-mass spectrometry-selected-ion monitoring provided a simple and sensitive method for analyzing amino acids in plasma and brain samples. Although the sensitivities of chemical ionization and electron-impact ionization were similar chemical ionization produced higher-mass ions, which might increase the selectivity of the assay. Both chemical and electron-impact ionization distinguished the natural amino acids from the 15N-labelled amino acids. The recovery of amino acids from plasma and brain samples was ca. 75%. The amino acid levels determined by gas chromatography-mass spectrometry were comparable with the amino acid levels determined by high-performance liquid chromatography or amino acid analyzer.
气相色谱-质谱联用选择离子监测法为分析血浆和脑样本中的氨基酸提供了一种简单且灵敏的方法。尽管化学电离和电子轰击电离的灵敏度相似，但化学电离产生的离子质量更高，这可能会提高测定的选择性。化学电离和电子轰击电离都能区分天然氨基酸和15N标记的氨基酸。血浆和脑样本中氨基酸的回收率约为75%。气相色谱-质谱联用测定的氨基酸水平与高效液相色谱法或氨基酸分析仪测定的氨基酸水平相当。
