Chen T L, Grochow L B, Hurowitz L A, Brundrett R B
John Hopkins Oncology Center, Johns Hopkins University, School of Medicine, Baltimore, MD 21205.
J Chromatogr. 1988 Mar 18;425(2):303-9. doi: 10.1016/0378-4347(88)80034-3.
A simple and highly sensitive gas chromatographic method has been developed for the determination of busulfan in human plasma. After extraction of plasma specimens (clinical or spiked) with ethyl acetate, busulfan and the internal standard [1,8-bis(methanesulfonyloxy)octane] were derivatized with 2,3,5,6-tetrafluorothiophenol to yield compounds monitored by a 63Ni electron-capture detector. Sample recoveries from extraction and derivatization were greater than 78 and 91%, respectively. The limit of quantitation was 0.01 microgram/ml (0.04 microM) in 1 ml of plasma with a linear relationship over the 0.01-1.0 micrograms/ml (0.04-4 microM) concentration range. The method has been applied to analyze the plasma versus time profile of busulfan in human subjects following administration of an oral dose of 4 mg/kg per day as a marrow ablative chemotherapy for bone marrow transplantation.
已开发出一种简单且高灵敏度的气相色谱法用于测定人血浆中的白消安。用乙酸乙酯萃取血浆标本（临床标本或加标标本）后，白消安和内标物[1,8 - 双（甲磺酰氧基）辛烷]用2,3,5,6 - 四氟硫酚进行衍生化，生成可由⁶³Ni电子捕获检测器监测的化合物。萃取和衍生化的样品回收率分别大于78%和91%。在1 ml血浆中定量限为0.01微克/毫升（0.04微摩尔/升），在0.01 - 1.0微克/毫升（0.04 - 4微摩尔/升）浓度范围内呈线性关系。该方法已应用于分析作为骨髓移植的骨髓清除化疗，口服剂量为每天4毫克/千克的人体受试者中白消安的血浆浓度随时间变化的情况。
