Jinno K, Nagoshi T, Tanaka N, Okamoto M, Fetzer J C, Biggs W R
School of Materials Science, Toyohashi University of Technology, Japan.
J Chromatogr. 1988 Jan 29;436(1):1-10. doi: 10.1016/s0021-9673(00)94558-8.
The effect of column temperature on the reversed-phase retention of polycyclic aromatic hydrocarbons has been investigated using various chemically bonded phases. Four solutes, coronene, tetrabenzo[a,cd,j,lm]perylene, tetrabenzo[a,cd,f,lm]perylene and benzo[lm]phenanthro[4,5,6-abcd]perylene, were used as the test probes. The temperature dependences of the retention are almost linear (the logarithm of capacity factor is proportional to the reciprocal of the column absolute temperature) with monomeric C18, monomeric C18 with endcapping and diphenyl bonded phases, while non-linear behaviour was observed with polymeric C18 phases. These differences in behaviour of the stationary phases are interpreted in terms of their structural differences.
使用各种化学键合相研究了柱温对多环芳烃反相保留的影响。使用了四种溶质，即晕苯、四苯并[a,cd,j,lm]苝、四苯并[a,cd,f,lm]苝和苯并[lm]菲并[4,5,6-abcd]苝作为测试探针。对于单体C18、封端的单体C18和二苯基键合相，保留的温度依赖性几乎呈线性（容量因子的对数与柱绝对温度的倒数成正比），而对于聚合C18相则观察到非线性行为。固定相行为的这些差异根据其结构差异进行了解释。
